8-Oxopurines (8-oxodG and 8-oxodA) and formamidopyrimidines (FaPydG and FaPydA) are major oxidative DNA lesions involved in cancer development and aging. Their mutagenicity is believed to result from a conformational shift of the N9-C1′ glycosidic bonds from anti to syn, which allows the lesions to form noncanonical Hoogsteen-type base pairs with incoming triphosphates during DNA replication. Here we present biochemical data and what are to our knowledge the first crystal structures of carbocyclic FaPydA and FaPydG containing DNA in complex with a high-fidelity polymerase. Crystallographic snapshots show that the cFaPy lesions keep the anti geometry of the glycosidic bond during error-free and error-prone replication. The observed dG·dC→dT·dA transversion mutations are the result of base shifting and tautomerization.
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We are grateful to the beamline scientists at the Swiss Light Source and European Synchrotron Radiation Facility for setting up the beamlines. This research project was supported by the Deutsche Forschungsgemeinschaft through SFB 646 and SFB 749. Further support was obtained by the Volkswagen Foundation and in particular by the Excellence Cluster CiPSM. We thank K. Karaghiosoff and K. Lux for solving the crystal structures of the small molecules. We thank M. Müller for critical reading of the manuscript and many helpful discussions.
The authors declare no competing financial interests.
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Gehrke, T., Lischke, U., Gasteiger, K. et al. Unexpected non-Hoogsteen–based mutagenicity mechanism of FaPy-DNA lesions. Nat Chem Biol 9, 455–461 (2013). https://doi.org/10.1038/nchembio.1254
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