Figure 2 : Formation of CCMV–dendron complexes.

From: Self-assembly and optically triggered disassembly of hierarchical dendron–virus complexes

Figure 2

a, DLS profiles showing the increase in the intensity-averaged hydrodynamic radius (Dh) when G1 (2) is titrated into a CCMV solution. Inset: corresponding second-order correlation functions. b,c, DLS data for the titrations of CCMV with G0 (1), G1 (2) and G2 (3) in the presence of 10 mM NaCl (b) and 150 mM NaCl (c), showing a decrease in the scattering intensity of the free virus (black symbols, primary axis) and formation of larger secondary assemblies (green symbols, secondary axis). Dendrons are able to assemble the virus particles in a generation-dependent manner, with the larger dendrons being more efficient. When the NaCl concentration is increased, a higher dendron concentration is also needed to assemble the virus particles. d, Variable-temperature CD spectra of CCMV between 20 °C and 90 °C showing a transition at 77 °C. e, Thermal denaturation of CCMV in the presence of G0 (1), G1 (2) and G2 (3) monitored by CD at 208 nm in the presence of 150 mM NaCl. Thermal stability of the virus is decreased in the presence of a dendron. f, Agarose gel electrophoresis and ζ-potential values of CCMV and complexes. On lanes 1 and 2 the CCMV particles are migrating freely. When dendron is added the free migration is disturbed due to complex formation (lanes 3 and 4). After UV exposure, the CCMV particles regain their electrophoretic mobility.