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Mass spectrometry

Raw protein from the top down

Mass spectrometry is a powerful technique for analysing proteins, yet linking higher-order protein structure to amino acid sequence and post-translational modifications is far from simple. Now, a native top-down method has been developed that can provide information on higher-order protein structure and different proteoforms at the same time.

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Figure 1: Native top-down mass spectrometry of protein complexes.

References

  1. 1

    Altelaar, A. F., Munoz, J. & Heck, A. J. Nat. Rev. Genet. 14, 35–48 (2013).

    CAS  Article  Google Scholar 

  2. 2

    Aebersold, R. & Mann, M. Nature 537, 347–355 (2016).

    CAS  Article  Google Scholar 

  3. 3

    Li, H., Nguyen, H. H., Loo, R. R. O., Campuzano, I. D. G. & Loo, J. A. Nat. Chem. 10, 139–148 (2018).

    CAS  Article  Google Scholar 

  4. 4

    Smith, L. M., Kelleher, N. L. & The Consortium for Top Down Proteomics Nat. Methods 10, 186–187 (2013).

    CAS  Article  Google Scholar 

  5. 5

    Catherman, A. D., Skinner, O. S. & Kelleher, N. L. Biochem. Biophys. Res. Commun. 445, 683–693 (2014).

    CAS  Article  Google Scholar 

  6. 6

    Brodbelt, J. S. Anal. Chem. 88, 30–51 (2016).

    CAS  Article  Google Scholar 

  7. 7

    Loo, J. A. Int. J. Mass Spectrom. 200, 175–186 (2000).

    CAS  Article  Google Scholar 

  8. 8

    Sharon, M. & Robinson, C. V. Annu. Rev. Biochem. 76, 167–193 (2007).

    CAS  Article  Google Scholar 

  9. 9

    Erba, E. B. & Petosa, C. Protein Sci. 24, 1176–1192 (2015).

    Article  Google Scholar 

  10. 10

    Lossl, P., van de Waterbeemd, M. & Heck, A. J. R. EMBO J. 35, 2634–2657 (2016).

    Article  Google Scholar 

  11. 11

    Breuker, K. & McLafferty, F. W. Proc. Natl Acad. Sci. USA 105, 18145–18152 (2008).

    CAS  Article  Google Scholar 

  12. 12

    Skinner, O. S. et al. Nat. Chem. Biol. 14, 36–41 (2018).

    CAS  Article  Google Scholar 

Download references

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Correspondence to Kathrin Breuker.

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Breuker, K. Raw protein from the top down. Nature Chem 10, 114–116 (2018). https://doi.org/10.1038/nchem.2936

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