Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Mass spectrometry

Raw protein from the top down

Mass spectrometry is a powerful technique for analysing proteins, yet linking higher-order protein structure to amino acid sequence and post-translational modifications is far from simple. Now, a native top-down method has been developed that can provide information on higher-order protein structure and different proteoforms at the same time.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Native top-down mass spectrometry of protein complexes.

References

  1. Altelaar, A. F., Munoz, J. & Heck, A. J. Nat. Rev. Genet. 14, 35–48 (2013).

    Article  CAS  Google Scholar 

  2. Aebersold, R. & Mann, M. Nature 537, 347–355 (2016).

    Article  CAS  Google Scholar 

  3. Li, H., Nguyen, H. H., Loo, R. R. O., Campuzano, I. D. G. & Loo, J. A. Nat. Chem. 10, 139–148 (2018).

    Article  CAS  Google Scholar 

  4. Smith, L. M., Kelleher, N. L. & The Consortium for Top Down Proteomics Nat. Methods 10, 186–187 (2013).

    Article  CAS  Google Scholar 

  5. Catherman, A. D., Skinner, O. S. & Kelleher, N. L. Biochem. Biophys. Res. Commun. 445, 683–693 (2014).

    Article  CAS  Google Scholar 

  6. Brodbelt, J. S. Anal. Chem. 88, 30–51 (2016).

    Article  CAS  Google Scholar 

  7. Loo, J. A. Int. J. Mass Spectrom. 200, 175–186 (2000).

    Article  CAS  Google Scholar 

  8. Sharon, M. & Robinson, C. V. Annu. Rev. Biochem. 76, 167–193 (2007).

    Article  CAS  Google Scholar 

  9. Erba, E. B. & Petosa, C. Protein Sci. 24, 1176–1192 (2015).

    Article  Google Scholar 

  10. Lossl, P., van de Waterbeemd, M. & Heck, A. J. R. EMBO J. 35, 2634–2657 (2016).

    Article  Google Scholar 

  11. Breuker, K. & McLafferty, F. W. Proc. Natl Acad. Sci. USA 105, 18145–18152 (2008).

    Article  CAS  Google Scholar 

  12. Skinner, O. S. et al. Nat. Chem. Biol. 14, 36–41 (2018).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Kathrin Breuker.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Breuker, K. Raw protein from the top down. Nature Chem 10, 114–116 (2018). https://doi.org/10.1038/nchem.2936

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nchem.2936

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing