Abstract
Bacterial meroterpenoids constitute an important class of natural products with diverse biological properties and therapeutic potential. The biosynthetic logic for their production is unknown and defies explanation via classical biochemical paradigms. A large subgroup of naphthoquinone-based meroterpenoids exhibits a substitution pattern of the polyketide-derived aromatic core that seemingly contradicts the established reactivity pattern of polyketide phenol nucleophiles and terpene diphosphate electrophiles. We report the discovery of a hitherto unprecedented enzyme-promoted α-hydroxyketone rearrangement catalysed by vanadium-dependent haloperoxidases to account for these discrepancies in the merochlorin and napyradiomycin class of meroterpenoid antibiotics, and we demonstrate that the α-hydroxyketone rearrangement is potentially a conserved biosynthetic reaction in this molecular class. The biosynthetic α-hydroxyketone rearrangement was applied in a concise total synthesis of naphthomevalin, a prominent member of the napyradiomycin meroterpenes, and sheds further light on the mechanism of this unifying enzymatic transformation.
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References
Kaysser, L. et al. Merochlorins A–D, cyclic meroterpenoid antibiotics biosynthesized in divergent pathways with vanadium-dependent chloroperoxidases. J. Am. Chem. Soc. 134, 11988–11991 (2012).
Wu, Z. et al. Antibacterial and cytotoxic new napyradiomycins from the marine-derived Streptomyces sp. SCSIO 10428. Mar. Drugs 11, 2113–2125 (2013).
Cheng, Y.-B., Jensen, P. R. & Fenical, W. Cytotoxic and antimicrobial napyradiomycins from two marine-derived Streptomyces strains. Eur. J. Org. Chem. 2013, 3751–3757 (2013).
Shiomi, K. et al. Novel antibiotics napyradiomycins. Production, isolation, physico-chemical properties and biological activity. J. Antibiot. 39, 487–493 (1986).
Haste, N. M. et al. Bactericidal kinetics of marine-derived napyradiomycins against contemporary methicillin-resistant Staphylococcus aureus. Mar. Drugs 9, 680–689 (2011).
Soria-Mercado, I. E., Prieto-Davó, A., Jensen, P. R. & Fenical, W. Antibiotic terpenoid chloro-dihydroquinones from a new marine actinomycete. J. Nat. Prod. 68, 904–910 (2005).
Farnaes, L. et al. Napyradiomycin derivatives, produced by a marine-derived actinomycete, illustrate cytotoxicity by induction of apoptosis. J. Nat. Prod. 77, 15–21 (2014).
Shin-ya, K. et al. Isolation and structural elucidation of an antioxidative agent, naphterpin. J. Antibiot. 43, 444–447 (1990).
Izumikawa, M., Nagai, A., Hashimoto, J., Takagi, M. & Shin-ya, K. Isolation of 2 new naphthablin analogs, JBIR-79 and JBIR-80, from Streptomyces sp. RI24. J. Antibiot. 63, 729–731 (2010).
Heide, L. Prenyl transfer to aromatic substrates: genetics and enzymology. Curr. Opin. Chem. Biol. 13, 171–179 (2009).
Tello, M., Kuzuyama, T., Heide, L., Noel, J. P. & Richard, S. B. The ABBA family of aromatic prenyltransferases: broadening natural product diversity. Cell. Mol. Life Sci. 65, 1459–1463 (2008).
Bach, T. J. & Rohmer, M. Isoprenoid Synthesis in Plants and Microorganisms: New Concepts and Experimental Approaches (Springer, 2012).
Matsuda, Y. & Abe, I. Biosynthesis of fungal meroterpenoids. Nat. Prod. Rep. 33, 26–53 (2015).
Xu, Z., Baunach, M., Ding, L. & Hertweck, C. Bacterial synthesis of diverse indole terpene alkaloids by an unparalleled cyclization sequence. Angew. Chem. Int. Ed. 51, 10293–10297 (2012).
Yamada, Y. et al. Terpene synthases are widely distributed in bacteria. Proc. Natl Acad. Sci. USA 112, 857–862 (2015).
Baunach, M., Franke, J. & Hertweck, C. Terpenoid biosynthesis off the beaten track: unconventional cyclases and their impact on biomimetic synthesis. Angew. Chem. Int. Ed. 54, 2604–2626 (2014).
Henkel, T. & Zeeck, A. Secondary metabolites by chemical screening, 15. Structure and absolute configuration of naphthomevalin, a new dihydro-naphthoquinone antibiotic from Streptomyces sp. J. Antibiot. 44, 665–669 (1991).
Shiomi, K. et al. New antibiotic napyradiomycins A2 and B4 and stereochemistry of napyradiomycins. J. Antibiot. 40, 1213–1219 (1987).
Shiomi, K. et al. Structures of new antibiotics: napyradiomycins. J. Antibiot. 39, 494–501 (1986).
Kalaitzis, J. A., Hamano, Y., Nilsen, G. & Moore, B. S. Biosynthesis and structural revision of neomarinone. Org. Lett. 5, 4449–4452 (2003).
Shin-ya, K., Furihata, K., Hayakawa, Y. & Seto, H. Biosynthetic studies of naphterpin, a terpenoid metabolite of Streptomyces. Tetrahedron Lett. 31, 6025–6026 (1990).
Pepper, H. & George, J. The biosynthesis and biomimetic synthesis of merochlorins A and B. Synlett 26, 2485–2490 (2015).
Funayama, S., Ishibashi, M., Komiyama, K. & Omura, S. Biosynthesis of furaquinocins A and B. J. Org. Chem. 55, 1132–1133 (2002).
Funa, N. et al. A new pathway for polyketide synthesis in microorganisms. Nature 400, 897–899 (1999).
Kuzuyama, T., Noel, J. P. & Richard, S. B. Structural basis for the promiscuous biosynthetic prenylation of aromatic natural products. Nature 435, 983–987 (2005).
Kumano, T., Tomita, T., Nishiyama, M. & Kuzuyama, T. Functional characterization of the promiscuous prenyltransferase responsible for furaquinocin biosynthesis: identification of a physiological polyketide substrate and its prenylated reaction products. J. Biol. Chem. 285, 39663–39671 (2010).
Leipoldt, F. et al. Diversity of ABBA prenyltransferases in marine Streptomyces sp. CNQ-509: promiscuous enzymes for the biosynthesis of mixed terpenoid compounds. PLoS ONE 10, e0143237 (2015).
Sydor, P. K. et al. Regio- and stereodivergent antibiotic oxidative carbocyclizations catalysed by Rieske oxygenase-like enzymes. Nat. Chem. 3, 388–392 (2011).
Li, S. et al. Hapalindole/ambiguine biogenesis is mediated by a Cope rearrangement, C–C bond-forming cascade. J. Am. Chem. Soc. 137, 15366–15369 (2015).
Teufel, R. et al. One-pot enzymatic synthesis of merochlorin A and B. Angew. Chem. Int. Ed. 53, 11019–11022 (2014).
Diethelm, S., Teufel, R., Kaysser, L. & Moore, B. S. A multitasking vanadium-dependent chloroperoxidase as an inspiration for the chemical synthesis of the merochlorins. Angew. Chem. Int. Ed. 53, 11023–11026 (2014).
Pepper, H. P. & George, J. H. Biomimetic total synthesis of (±)-merochlorin A. Angew. Chem. Int. Ed. 52, 12170–12173 (2013).
Meier, R., Strych, S. & Trauner, D. Biomimetic synthesis of (±)-merochlorin B. Org. Lett. 16, 2634–2637 (2014).
Katsuyama, Y., Harmrolfs, K., Pistorius, D., Li, Y. & Müller, R. A semipinacol rearrangement directed by an enzymatic system featuring dual-function FAD-dependent monooxygenase. Angew. Chem. Int. Ed. 51, 9437–9440 (2012).
Tatsuta, K., Tanaka, Y., Kojima, M. & Ikegami, H. The first total synthesis of (±)-napyradiomycin A1. Chem. Lett. 14–14 (2002).
Snyder, S. A., Tang, Z.-Y. & Gupta, R. Enantioselective total synthesis of (–)-napyradiomycin A1 via asymmetric chlorination of an isolated olefin. J. Am. Chem. Soc. 131, 5744–5745 (2009).
Takemura, S. et al. A concise total synthesis of (±)-A80915G, a member of the napyradiomycin family of antibiotics. Tetrahedron Lett. 40, 7501–7505 (1999).
Beekman, A. M., Castillo Martinez, E. & Barrow, R. A. First syntheses of the biologically active fungal metabolites pestalotiopsones A, B, C and F. Org. Biomol. Chem. 11, 1109–1115 (2013).
Kimura, M., Fukasaka, M. & Tamaru, Y. Palladium-catalyzed, triethylborane-promoted C-allylation of naphthols and benzene polyols by direct use of allyl alcohols. Synthesis 2006, 3611–3616 (2006).
Roche, S. P. & Porco, J. A. Jr . Dearomatization strategies in the synthesis of complex natural products. Angew. Chem. Int. Ed. 50, 4068–4093 (2011).
Essa, A. H. et al. Reduction of 2,2,2-trichloro-1-arylethanones by RMgX: mechanistic investigation and the synthesis of substituted α,α-dichloroketones. Chem. Commun. 49, 2756–2758 (2013).
Narayan, S. et al. ‘On water’: unique reactivity of organic compounds in aqueous suspension. Angew. Chem. Int. Ed. 44, 3275–3279 (2005).
Katsuyama, Y., Li, X.-W., Müller, R. & Nay, B. Chemically unprecedented biocatalytic (AuaG) retro-[2,3]-Wittig rearrangement: a new insight into aurachin B biosynthesis. Chembiochem 15, 2349–2352 (2014).
Winter, J. M. et al. Molecular basis for chloronium-mediated meroterpene cyclization: cloning, sequencing, and heterologous expression of the napyradiomycin biosynthetic gene cluster. J. Biol. Chem. 282, 16362–16368 (2007).
Bernhardt, P., Okino, T., Winter, J. M., Miyanaga, A. & Moore, B. S. A stereoselective vanadium-dependent chloroperoxidase in bacterial antibiotic biosynthesis. J. Am. Chem. Soc. 133, 4268–4270 (2011).
Winter, J. M. & Moore, B. S. Exploring the chemistry and biology of vanadium-dependent haloperoxidases. J. Biol. Chem. 284, 18577–18581 (2009).
Agarwal, V. et al. Enzymatic halogenation and dehalogenation reactions: pervasive and mechanistically diverse. Chem. Rev. 117, 5619–5674 (2017).
Soedjak, H. S., Walker, J. V. & Butler, A. Inhibition and inactivation of vanadium bromoperoxidase by the substrate hydrogen peroxide and further mechanistic studies. Biochemistry 34, 12689–12696 (1995).
de Boer, E. & Wever, R. The reaction mechanism of the novel vanadium-bromoperoxidase. A steady-state kinetic analysis. J. Biol. Chem. 263, 12326–12332 (1988).
Acknowledgements
We are grateful to our University of California San Diego colleagues B. Duggan for assistance with NMR measurements and X. Tang for helpful discussions. We are also grateful to M. Ghadiri and L. J. Leman at The Scripps Research Institute for their help in the collection of CD measurements. S.D. acknowledges the Swiss National Science Foundation for a postdoctoral fellowship. This research was supported by the US National Institutes of Health (R01-AI047818) and the Australian Research Council (DP160103393), and was undertaken with the assistance of resources from the National Computational Infrastructure, which is supported by the Australian Government.
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Z.D.M., S.D., H.P.P., J.H.G. and B.S.M. designed the study and wrote the manuscript with input from all of the authors. Z.D.M., S.D. and H.P.P. performed the experiments. D.M.H. performed the computational studies.
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Miles, Z., Diethelm, S., Pepper, H. et al. A unifying paradigm for naphthoquinone-based meroterpenoid (bio)synthesis. Nature Chem 9, 1235–1242 (2017). https://doi.org/10.1038/nchem.2829
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DOI: https://doi.org/10.1038/nchem.2829
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