Analysing post-translational modifications of histone proteins as they occur within chromatin is challenging due to their large number and chemical diversity. A major step forward has now been achieved by using split intein chemistry to engineer functionalized histones within cells.
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Fischle, W., Schwarzer, D. & Mootz, H. Chromatin chemistry goes cellular. Nature Chem 7, 371–373 (2015). https://doi.org/10.1038/nchem.2249
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DOI: https://doi.org/10.1038/nchem.2249
