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Fluorescence microscopy

Strategic blinking

For decades chemists have focused on increasing the brightness of fluorophores. In super-resolution microscopy, however, fluorophores that preferentially exist in a non-fluorescent state, but occasionally re-arrange into a fluorescent form, can give better results.

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Figure 1: Construction of super-resolution images using the hydroxymethyl silicon-rhodamine (HMSiR) dye.


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Correspondence to Kai Johnsson.

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Lukinavičius, G., Johnsson, K. Strategic blinking. Nature Chem 6, 663–664 (2014).

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