Labelling of proteins with pairs of fluorophores enables their conformations to be studied; however, complete incorporation of labels in multiple, pre-defined locations is very difficult. Now, a combination of double unnatural amino acid mutagenesis and selective chemical modification offers a general method to achieve this.
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Petersson, E., Warner, J. Two-for-one designer labels. Nature Chem 6, 379–381 (2014). https://doi.org/10.1038/nchem.1935