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Synthetic biology

Two-for-one designer labels

Labelling of proteins with pairs of fluorophores enables their conformations to be studied; however, complete incorporation of labels in multiple, pre-defined locations is very difficult. Now, a combination of double unnatural amino acid mutagenesis and selective chemical modification offers a general method to achieve this.

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Figure 1: Efficient double labelling of a protein for FRET studies is made possible by orthogonal machinery for unnatural amino acid incorporation and orthogonal chemical reactions.


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Correspondence to E. James Petersson.

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Petersson, E., Warner, J. Two-for-one designer labels. Nature Chem 6, 379–381 (2014).

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