Perspective

Opportunities and challenges in single-molecule and single-particle fluorescence microscopy for mechanistic studies of chemical reactions

  • Nature Chemistry volume 5, pages 993999 (2013)
  • doi:10.1038/nchem.1800
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Abstract

In recent years, single-molecule and single-particle fluorescence microscopy has emerged as a tool to investigate chemical systems. After an initial lag of over a decade with respect to biophysical studies, this powerful imaging technique is now revealing mechanisms of 'classical' organic reactions, spatial distribution of chemical reactivity on surfaces and the phase of active catalysts. The recent advance into commercial imaging systems obviates the need for home-built laser systems and thus opens this technique to traditionally trained synthetic chemists. We discuss the requisite photophysical and chemical properties of fluorescent reporters and highlight the main challenges in applying single-molecule techniques to chemical questions. The goal of this Perspective is to provide a snapshot of an emerging multidisciplinary field and to encourage broader use of this young experimental approach that aids the observation of chemical reactions as depicted in many textbooks: molecule by molecule.

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Acknowledgements

T.C. was supported by the Zernike Institute for Advanced Materials and the Centre for Synthetic Biology (University of Groningen). S.A.B. thanks the US Department of Energy, Office of Basic Energy Sciences for support (DE-FG02-08ER15994).

Author information

Affiliations

  1. Molecular Microscopy Research Group and Single-molecule Biophysics, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands

    • Thorben Cordes
  2. Department of Chemistry, University of California, Irvine, California 92697-2025, USA

    • Suzanne A. Blum

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Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Thorben Cordes or Suzanne A. Blum.