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An actin nucleation mechanism mediated by Bni1 and Profilin

Nature Cell Biology volume 4, pages 626631 (2002) | Download Citation

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Abstract

Formins are required for cell polarization and cytokinesis, but do not have a defined biochemical activity. In Saccharomyces cerevisiae, formins and the actin-monomer-binding protein profilin are specifically required to assemble linear actin structures called 'actin cables'. These structures seem to be assembled independently of the Arp2/3 complex, the only well characterized cellular mediator of actin nucleation. Here, an activated yeast formin was purified and found to promote the nucleation of actin filaments in vitro. Formin-dependent actin nucleation was stimulated by profilin. Thus, formin and profilin mediate actin nucleation by an Arp2/3-independent mechanism. These findings suggest that distinct actin nucleation mechanisms may underlie the assembly of different actin cytoskeletal structures.

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References

  1. 1.

    , & Annu. Rev. Biophys. Biomol. Struct. 29, 545–576 (2000).

  2. 2.

    Trends Cell Biol. 11, 288–293 (2001).

  3. 3.

    & Annu. Rev. Biochem. 70, 649–676 (2001).

  4. 4.

    , , , & Nature Cell Biol. 4, 32–41 (2002).

  5. 5.

    & J. Cell Biol. 156, 677–687 (2002).

  6. 6.

    Nature Cell Biol. 4, E29–E30 (2002).

  7. 7.

    , & Nature Cell Biol. 4, 42–50 (2002).

  8. 8.

    , & Proc. Natl Acad. Sci. USA 96, 7288–7293 (1999).

  9. 9.

    & Curr. Biol. 11, 1656–1665 (2001).

  10. 10.

    & Trends Cell Biol. 11, 38–46 (2001).

  11. 11.

    et al. J. Cell Biol. 110, 105–114 (1990).

  12. 12.

    et al. Mol. Cell. Biol. 21, 827–839 (2001).

  13. 13.

    & Biochemistry 30, 1973–1980 (1991).

  14. 14.

    & Cell 20, 329–341 (1980).

  15. 15.

    & Biochemistry 23, 6631–6641 (1984).

  16. 16.

    & Biochemistry 37, 13276–13284 (1998).

  17. 17.

    et al. Proc. Natl Acad. Sci. USA 96, 3739–3744 (1999).

  18. 18.

    & Mol. Biol. Cell 12, 1161–1175 (2001).

  19. 19.

    , , , & J. Cell Biol. 150, 895–904 (2000).

  20. 20.

    , & Biochemistry 38, 15212–15222 (1999).

  21. 21.

    , , , & Nature Cell Biol. 2, 385–391 (2000).

  22. 22.

    et al. Science online (cited 06 June 2002) DOI: 10.1126/science.1072309.

  23. 23.

    & J. Biol. Chem. 261, 12754–12758 (1986).

  24. 24.

    et al. Mol. Biol. Cell 10, 2481–2491 (1999).

  25. 25.

    et al. Mol. Cell 5, 13–25 (2000).

  26. 26.

    , , , & Nature Cell Biol. 1, 136–143 (1999).

  27. 27.

    et al. J. Cell Biol. 144, 83–98 (1999).

  28. 28.

    , , & J. Cell Biol. 153, 627–634 (2001).

  29. 29.

    , & Curr. Biol. 9, 501–504 (1999).

  30. 30.

    J. Biol. Chem. 276, 2824–2830 (2001).

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Acknowledgements

We thank H. Balcer, C. Boone, D. Drubin, A. Lasek, and A. Manning for reagents; P. Carvalho, T. Fujiwara, M.E. McLaughlin and T. Rapoport for comments on the manuscript; H. Balcer, P. Carvalho, H. Falet, J. Hartwig and T. Rapoport for discussions. This work was supported by grants from the Pew Scholars Program (B.G.); a Basil O'Connor Scholar Award (B.G.); a pre-doctoral fellowship from the Howard Hughes Medical Institute (A.R.); the National Institutes of Health (GM61345, D.P.); the Leukemia and Lymphoma Society of America (D.P.).

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Affiliations

  1. Departments of Pediatric Oncology, The Dana-Farber Cancer Institute and Pediatric Hematology, The Children's Hospital, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA

    • Isabelle Sagot
    •  & David Pellman
  2. Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720-3202, USA

    • Avital A. Rodal
  3. Department of Biology, Rosenstiel Medical Center, Brandeis University, 415 South Street, Waltham, MA 02454, USA

    • James Moseley
    •  & Bruce L. Goode

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The authors declare no competing financial interests.

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DOI

https://doi.org/10.1038/ncb834

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