Abstract
Regulation of the actin cytoskeleton by microtubules is mediated by the Rho family GTPases. However, the molecular mechanisms that link microtubule dynamics to Rho GTPases have not, as yet, been identified. Here we show that the Rho guanine nucleotide exchange factor (GEF)-H1 is regulated by an interaction with microtubules. GEF-H1 mutants that are deficient in microtubule binding have higher activity levels than microtubule–bound forms. These mutants also induce Rho-dependent changes in cell morphology and actin organization. Furthermore, drug-induced microtubule depolymerization induces changes in cell morphology and gene expression that are similar to the changes induced by the expression of active forms of GEF-H1. Furthermore, these effects are inhibited by dominant-negative versions of GEF-H1. Thus, GEF-H1 links changes in microtubule integrity to Rho-dependent regulation of the actin cytoskeleton.
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Acknowledgements
The authors are grateful to C. Waterman-Storer and T. Wittmann for helpful discussions, to B. Bohl for help in preparation of recombinant proteins, and to B. Fowler for assistance with preparation of GEF-H1 mutants. This work was supported by a United States Public Health Service grant GM39434 to G.M.B., an Arthritis Foundation Postdoctoral Fellowship to M.K., and Fellowship DAMD17-98-1-8151 from the United States Army Breast Cancer Research Program to F.T.Z.
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Figure S1
Cos-1 cells were transfected with SRE-luciferase and LacZ reporter plasmids and varying amounts of GEF-H1 construct lacking DH and PH domains (GEFH1delDHPH). (PDF 22 kb)
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Krendel, M., Zenke, F. & Bokoch, G. Nucleotide exchange factor GEF-H1 mediates cross-talk between microtubules and the actin cytoskeleton. Nat Cell Biol 4, 294–301 (2002). https://doi.org/10.1038/ncb773
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DOI: https://doi.org/10.1038/ncb773
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