a) At collection for Hi-C analysis an aliquot of cells was fixed and DNA stained with DAPI. Only experiments that had >94% large budded cells were taken for further processing. Budding index demonstrates CDK activation in yeast cells. Mitotic cells were then assessed as to whether they had maintained the pre-anaphase arrest—as indicated by a single nucleus. Or had proceeded into anaphase—as indicated by 2 split nuclei. Abbreviations for states are as used in text specifically M - cdc20 arrested, MH—cohesin depleted ( scc1-73) cdc20 arrested, MD – condensin depleted ( smc2td GAL1-smc2K38I) cdc20 arrested. R1 and R2 refer to replicate 1 and replicate 2, respectively. Therefore two independent experiments were conducted for each state. ( b) Telomeres (40 kb) of all chromosome arms have been grouped according to arm length. The interaction frequency between the 8 shortest and 8 longest arms relative to each other has been analyzed. ( c) Zoom into contact heatmaps from G1 and M datasets for selected regions on (top) ChrXV (0-330 kb (CENXV is at 330 kb)) and (bottom) the post-rDNA region of ChrXII (660 kb to 940 kb). Each block represents 10 kb bin. ( d) Zoom into log2 ratio of M over G1 contact maps for selected regions on (top) ChrXV (0-330 kb (CENXV is at 330 kb)) and (bottom) the post-rDNA region of ChrXII (660 kb to 940 kb). ( e) Overlaid P( s) curves for each individual chromosome arm taken from G1 (green) or M (blue) cells. All contact maps shown were assembled from two independent experiments.