Supplementary Figure 1 : Experimental set up and confirmation of Rabl conformation of yeast nucleus arrested in G1 and M.

From: SMC complexes differentially compact mitotic chromosomes according to genomic context

Supplementary Figure 1

(a) At collection for Hi-C analysis an aliquot of cells was fixed and DNA stained with DAPI. Only experiments that had >94% large budded cells were taken for further processing. Budding index demonstrates CDK activation in yeast cells. Mitotic cells were then assessed as to whether they had maintained the pre-anaphase arrest—as indicated by a single nucleus. Or had proceeded into anaphase—as indicated by 2 split nuclei. Abbreviations for states are as used in text specifically M - cdc20 arrested, MH—cohesin depleted (scc1-73) cdc20 arrested, MD – condensin depleted (smc2td GAL1-smc2K38I) cdc20 arrested. R1 and R2 refer to replicate 1 and replicate 2, respectively. Therefore two independent experiments were conducted for each state. (b) Telomeres (40 kb) of all chromosome arms have been grouped according to arm length. The interaction frequency between the 8 shortest and 8 longest arms relative to each other has been analyzed. (c) Zoom into contact heatmaps from G1 and M datasets for selected regions on (top) ChrXV (0-330 kb (CENXV is at 330 kb)) and (bottom) the post-rDNA region of ChrXII (660 kb to 940 kb). Each block represents 10 kb bin. (d) Zoom into log2 ratio of M over G1 contact maps for selected regions on (top) ChrXV (0-330 kb (CENXV is at 330 kb)) and (bottom) the post-rDNA region of ChrXII (660 kb to 940 kb). (e) Overlaid P(s) curves for each individual chromosome arm taken from G1 (green) or M (blue) cells. All contact maps shown were assembled from two independent experiments.