Supplementary Figure 5: (Related to Fig. 7 and 8). | Nature Cell Biology

Supplementary Figure 5: (Related to Fig. 7 and 8).

From: Pramel7 mediates ground-state pluripotency through proteasomal–epigenetic combined pathways

Supplementary Figure 5

(a) Representative images of ESCs grown in 2i condition and upon 5 days of differentiation. Upon differentiation methylation defective Uhrf1−/− and DNMT-TKO ESCs formed less differentiated and more compact colonies as in the case of P7-ESCs. Scale bar: 200 μm. Representative of 2 independent experiments. (b) Expression of pluripotency-associated genes Nanog, Oct4 and Rex1 in three P7 reconverted clones. Values were measured by qRT-PCR, normalized to actin mRNA and represented as fold change relative to the expression in E14-ESCs. Average values of two independent experiments.

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