(a) Representative images of ESCs grown in 2i condition and upon 5 days of differentiation. Upon differentiation methylation defective Uhrf1−/− and DNMT-TKO ESCs formed less differentiated and more compact colonies as in the case of P7-ESCs. Scale bar: 200 μm. Representative of 2 independent experiments. (b) Expression of pluripotency-associated genes Nanog, Oct4 and Rex1 in three P7 reconverted clones. Values were measured by qRT-PCR, normalized to actin mRNA and represented as fold change relative to the expression in E14-ESCs. Average values of two independent experiments.