(a) Oxygen bomb calorimetry revealed similar caloric values in MG1655LB and MG1655M9. (b,c) The levels of triacylglycerides (b) and proteins (c) were measured biochemically and found no significant differences between MG1655LB and MG1655M9. n.s. p > 0.05, Student’s t-test; n = 7 biologically independent experiments (b), n = 5 biologically independent experiments (c). (d) MG1655M9 has a higher level of carbohydrates than MG1655LB. ∗p < 0.05, Student’s t test; n = 6 biologically independent experiments. (e) Fermenting MG1655LB+glucose (with 0.2% glucose), as verified in (f), is not sufficient to recapitulate MG1655M9-conferred lipid accumulation in C. elegans. C. elegans on MG1655LB (without glucose) served as negative controls. n.s. p > 0.05, Student’s t-test; n = 3 biologically independent experiments. (f) In phenol red fermentation tests, MG1655M9 shows positive fermentation (yellow), but MG1655LB shows negative fermentation (red). Addition of glucose (0.2%) to LB is sufficient to drive MG1655LB+glucose to undergo fermentation. The experiments were independently replicated in laboratory 3 times with similar results. Error bars represent mean ± SEM.