Supplementary Figure 1: Physiological measurements in C. elegans living on MG1655LB and MG1655M9. | Nature Cell Biology

Supplementary Figure 1: Physiological measurements in C. elegans living on MG1655LB and MG1655M9.

From: Microbial metabolites regulate host lipid metabolism through NR5A–Hedgehog signalling

Supplementary Figure 1

(a) Adult C. elegans significantly increase or decrease fat content levels within 24 hours of switching to MG1655M9 or MG1655LB, respectively. p < 0.05, p < 0.01, Student’s t-test; n = 3 biologically independent experiments. (b,c) C. elegans raised on MG1655LB and on MG1655M9 show similar rates of pharyngeal pumping (b) and defecation (c), indicating comparable food intake rate and food retention time. Error bars represent standard deviation (SD). p > 0.05, Student’s t-test; n = 3 biologically independent experiments. (d) C. elegans raised on MG1655LB and MG1655M9 show indistinguishable rates of lipid absorption, assayed by a lipophilic BODIPY fluorescence dye. p > 0.05, Student’s t-test; n = 10 biologically independent animals. The box plots were generated to indicate ranges of min to max values, with 25th, 50th and 75th percentiles. (eg) C. elegans raised on MG1655LB and MG1655M9 show similar levels of mobility (e, p > 0.05, Student’s t-test; n = 11 biologically independent animals), lifespan (f, p > 0.05, Log-rank test; n = 100 biologically independent animals), and brood size (g, p > 0.05, Student’s t-test; n = 18 biologically independent animals). The experiments were independently replicated in laboratory 3 times with similar results. (h) C. elegans raised on E. coli cultured in M9 medium supplemented with either peptone or casamino acids (CAA) show reduced lipid accumulation, compared with those on MG1655M9p < 0.01, p < 0.001, Student’s t-test; n = 5 biologically independent experiments. (i) With bacteria killed by carbenicillin (60 μg/ml), MG1655M9-conferred lipid accumulation in C. elegans can still be significantly suppressed by supplementation of methionine but not betaine or homocysteine. p < 0.001, p < 0.01, n.s. p > 0.05, Student’s t-test. ##p < 0.01, n.s. p > 0.05, two-way ANOVA; n = 3 biologically independent experiments. (j) Mitochondrial DNA copy numbers are not significantly different among C. elegans raised on MG1655LB, MG1655LB+Methionine, MG1655M9, and MG1655M9+Methionine, n.s. p > 0.05, one-way ANOVA; n = 3 biologically independent experiments. (k) The fzo-1(tm1333) mutant exhibits completely fragmented mitochondrial morphology. p < 0.001, Chi-squared test; n = 50 biologically independent cells. The experiments were independently replicated in laboratory 3 times with similar results. Error bars represent mean ± standard error of the mean (SEM).

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