Supplementary Figure 4: Dense-core vesicle release from sensory neurons regulates MPK-1 activity in the germline. | Nature Cell Biology

Supplementary Figure 4: Dense-core vesicle release from sensory neurons regulates MPK-1 activity in the germline.

From: Insulin-like signalling to the maternal germline controls progeny response to osmotic stress

Supplementary Figure 4

(a) Representative germlines dissected from wild-type or unc-31(ft1) animals, which exhibit reduced dense-core vesicle release2, at 50 mM NaCl and stained for DNA (DAPI, white) and diphosphorylated MPK-1 (dpMPK-1) (red). SN::UNC-31(+) animals that expressed a rescuing copy of unc-31 specifically in sensory neurons using the osm-6 promoter. Experiment replicated 4 times. Scale bar 100 μm. (b) Percent of animals mobile and feeding after 48 h at 500 mM NaCl. Error bars s.d. n = 3 experiments of >100 animals. We note that the observation that less wild-type animals adapted to 500 mM NaCl when parents were exposed to 300 mM NaCl than in Fig. 2a is likely due to the fact that in this experiment embryos had to be collected by bleaching to remove any contaminating PA14 and this likely added an extra stressor to the embryos.

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