Supplementary Figure 1: C. elegans arrests development in response to osmotic stress. | Nature Cell Biology

Supplementary Figure 1: C. elegans arrests development in response to osmotic stress.

From: Insulin-like signalling to the maternal germline controls progeny response to osmotic stress

Supplementary Figure 1

(a) Representative images of wild-type animals after 72 h of exposure to 50 mM or 500 mM NaCl. Experiment replicated 12 times Scale bar 200 μm. (b) Percent of wild-type animals developing past the L1 larval stage after 24 h of exposure to 50 mM NaCl and 450 mM KCl. Error bars, s.d. n = 3 experiments of >100 animals. (c) Percent of wild-type animals developing past the L1 larval stage after 24 h of exposure to 50 mM NaCl and 450 mM NaBr. Error bars, s.d. n = 3 experiments of >100 animals. (d) Percent of wild-type animals developing past the L1 larval stage after 24 h of exposure to 50 mM NaCl and 900 mM sucrose. Sucrose was added at twice the concentration of salts to compensate for the difference in osmolarity. Error bars, s.d. n = 3 experiments of >100 animals. (e) Percent of wild-type, pmk-1(km25), or skn-1(zu67) animals developing past the L1 larval stage after 48 h of exposure to 300 mM NaCl. Error bars, s.d. n = 3 experiments of >100 animals. (f) Percent of wild-type, pmk-1(km25), or skn-1(zu67) animals failing to arrest development at 500 mM NaCl after 48 h. Error bars, s.d. n = 3 experiments of >100 animals. (g) Percent of wild-type and unc-31(ft1) animals failing to arrest development after 48 h. The osm-6 promoter was used to drive the expression of UNC-31 specifically in sensory neurons. Error bars, s.d. n = 3 experiments of >100 animals. (h) Percent of wild-type and ins-3(tm3608) mutant animals failing to arrest development at 400 mM NaCl after 48 h. Error bars, s.d. n = 3 experiments of >100 animals. (i) Percent of wild-type, ins-3(ok2488), ins-3(ok2488);daf-2(e1370) and rescue animals failing to arrest development at 400 mM NaCl 48 h post-hatching. Error bars, s.d. n = 3 experiments of >100 animals. The variation between the wild type in panels (a) and (b) is likely to be a result of variations in evaporation between different batches of Petri plates. The quantified results are presented as mean ± s.d. using two-tailed t-test. P < 0.05, P < 0.01, P < 0.001, P < 0.0001 were considered significant. n.s., not significant. See Statistics Source Data in Supplementary Table 6.

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