Figure 3: Insulin-like signalling to the maternal germline modifies progeny response to osmotic stress by regulating the RAS–ERK-like pathway. | Nature Cell Biology

Figure 3: Insulin-like signalling to the maternal germline modifies progeny response to osmotic stress by regulating the RAS–ERK-like pathway.

From: Insulin-like signalling to the maternal germline controls progeny response to osmotic stress

Figure 3

(a) Percentage of wild-type and lin-45(n2018), mek-2(ku114), mpk-1(n5639) and lin-45(n2018); gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 h. Error bars, s.d. n = 6, 6, 3, 3, and 3 experiments of >100 animals. See Supplementary Table 6. (b) Percentage of wild-type and lin-45(n2018) cross progeny failing to arrest development at 500 mM NaCl after 48 h. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of >20 animals. (c) Representative germlines dissected from wild-type animals exposed to either 50 mM NaCl or 300 mM NaCl and stained for DNA (DAPI, white) and diphosphorylated MPK-1 (dpMPK-1) (red). Each condition was replicated 16 times. Asterisks, mitotic end of germline. (d) Relative expression of gpdh-2 mRNA in wild-type and lin-45(n2018) embryos at 50 mM NaCl measured by qRT–PCR and normalized to the expression of the histone his-24. Error bars, s.d. n = 3 experiments from pellets of >1,000 embryos. (e) Glycerol-to-glucose ratio in wild-type and lin-45(n2018) mutant embryos at 50 mM NaCl. Error bars, s.d. n = 3 experiments from pellets of >1,000 embryos. The quantified results are presented as mean ± s.d. using ANOVA (a, b) and two-tailed t-test (d, e). P < 0.05, P < 0.01, P < 0.001 were considered significant. NS, not significant. See Statistics Source Data in Supplementary Table 6.

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