Figure 2: Insulin-like signalling to the maternal germline regulates progeny response to osmotic stress. | Nature Cell Biology

Figure 2: Insulin-like signalling to the maternal germline regulates progeny response to osmotic stress.

From: Insulin-like signalling to the maternal germline controls progeny response to osmotic stress

Figure 2

(a) Percentage of wild-type (WT), daf-2(e1370) and daf-2(e1370); daf-16(mu86) cross progeny failing to arrest development after 48 h at 500 mM NaCl. Males contained (Pegl-1::4xNLS::GFP); him-5(e1490); nIs349 (Pceh-28::4xNLS::mCherry) for the identification of cross progeny. The pie-1 promoter was used to drive germline-specific expression of DAF-2 and the mex-5 promoter was used to drive germline-specific expression of DAF-16. Error bars, s.d. n = 7, 3, 6, 3, 3, 3 and 3; see Supplementary Table 6. (b) Percentage of wild-type, gpdh-1(ok1558) and gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 h. Error bars, s.d. n = 3 experiments of >100 animals. (c) Average fold change of 2 replicates of the 25 most upregulated genes in embryos in response to osmotic stress after 6 h. FPKM, fragments per kilobase of transcript per million mapped reads. (d) Percentage of wild-type, daf-2(e1370) and gpdh-2(ok1733) cross progeny failing to arrest development after 48 h at 500 mM NaCl. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of >20 animals. The quantified results are presented as mean ± s.d. using ANOVA. P < 0.05, P < 0.001, P < 0.0001 were considered significant. NS, not significant. See Statistics Source Data in Supplementary Table 6.

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