Figure 1: Insulin-like signalling to the intestine regulates developmental arrest in response to osmotic stress. | Nature Cell Biology

Figure 1: Insulin-like signalling to the intestine regulates developmental arrest in response to osmotic stress.

From: Insulin-like signalling to the maternal germline controls progeny response to osmotic stress

Figure 1

(a) Percentage of wild-type, daf-2(e1370), age-1(hx546), pdk-1(sa709), daf-16(mu86) and daf-2(e1370); daf-16(mu86) animals developing past the L1 larval stage (L2+) after 48 h. Error bars, s.d. n = 3 experiments of >100 animals; P < 0.01 for all genotypes (two-tailed t-test). (b) Percentage of wild-type, daf-2(e1370), daf-16(mu86) and daf-2(e1370); daf-16(mu86) animals that resume development after 24 h of exposure to osmotic stress. Error bars, s.d. n = 3; see Supplementary Table 6; P < 0.01 for all genotypes (two-tailed t-test). (c) Percentage of wild-type and daf-2(e1370) animals developing past the L1 larval stage at 300 mM NaCl after 48 h. Neuron-specific expression was driven by Prgef-1; intestine-specific expression was driven by Pges-1; muscle-specific expression was driven by Pmyo-3. Error bars, s.d. n = 3 experiments of >100 animals. (d) Confocal images of DAF-16::GFP after 6 h of exposure to 50 mM and 500 mM NaCl. Percentage values indicate the percentage of embryos resembling the representative image. See Supplementary Table 6. 3 experimental replicates of >100 animals. The quantified results are presented as mean ± s.d. using two-tailed t-test; P < 0.0001 were considered significant. See Statistics Source Data in Supplementary Table 6.

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