Supplementary Figure 4 : PEX5 contains ATM S*/T*Q phosphorylation motif.

From: ATM functions at the peroxisome to induce pexophagy in response to ROS

Supplementary Figure 4

(a) Prediction of PEX5 phosphorylation site and ubiquination site by ScanSite and PhosphoSite. (b) Conserved S141 (ATM phosphorylation site) sequence in PEX5. (c) WT fibroblasts (GM08399) and AT fibroblasts (GM05849) were transfected with Myc-PEX5-WT and treated with H2O2 for 1 h. The complex was immunoprecipitated with anti-Myc and immunoblotted with phospho-(S/T) ATM substrate antibody. Inputs were immunoblotted using the indicated antibodies. Uncropped images of western blots are shown in Supplementary Fig. 9.