(a) Movement trajectories (green) of fluorescent nanoparticles coated with myosin VI along the actin cytoskeleton (red) of a fixed extracted cell. The white box marks the view shown in b. Scale bar, 10 μm. (b–e) High magnification view of myosin VI nanoparticle movement along the single transverse fiber. (b) A view of actin cytoskeleton (red) after extraction. White-boxed region: The transverse fiber decorated with myosin VI nanoparticles (green) is marked by the dotted line. (c–e) Sequential images from the live imaging of myosin VI nanoparticles moving along the transverse fiber. Nanoparticles #1 and #2 (white arrowheads) are moving away from each other. Scale bars, 1 μm. The full-length sequence is shown in Supplementary Video 6.