The lung alveoli regenerate after surgical removal of the left lobe by pneumonectomy (PNX). How this alveolar regrowth/regeneration is initiated remains unknown. We found that platelets trigger lung regeneration by supplying stromal-cell-derived factor-1 (SDF-1, also known as CXCL12). After PNX, activated platelets stimulate SDF-1 receptors CXCR4 and CXCR7 on pulmonary capillary endothelial cells (PCECs) to deploy the angiocrine membrane-type metalloproteinase MMP14, stimulating alveolar epithelial cell (AEC) expansion and neo-alveolarization. In mice lacking platelets or platelet Sdf1, PNX-induced alveologenesis was diminished. Reciprocally, infusion of Sdf1+/+ but not Sdf1-deficient platelets rescued lung regeneration in platelet-depleted mice. Endothelial-specific ablation of Cxcr4 and Cxcr7 in adult mice similarly impeded lung regeneration. Notably, mice with endothelial-specific Mmp14 deletion exhibited impaired expansion of AECs but not PCECs after PNX, which was not rescued by platelet infusion. Therefore, platelets prime PCECs to initiate lung regeneration, extending beyond their haemostatic contribution. Therapeutic targeting of this haemo-vascular niche could enable regenerative therapy for lung diseases.
In mammals, surgical removal of the left lung by pneumonectomy (PNX) leads to restoration of mass and gas exchange function in the residual lung alveoli1,2,3,4,5,6,7,8. This process of compensatory lung regrowth/regeneration results from formation of new functional alveolar units. PNX-induced neo-alveolarization is driven by expansion of several cell types, which include alveolar epithelial cells (AECs; refs 9, 10, 11, 12, 13), endothelial cells1,14,15,16,17,18,19 and mesenchymal cells3. However, it remains unknown how the loss of the lung lobe triggers the alveolar regeneration in the remaining lungs.
Pulmonary capillary endothelial cells (PCECs) lining the lung microvasculature form a specialized vascular niche to modulate the function of alveolar progenitor cells11,12,13,14,15,16,17,18. Following PNX, upregulation of the membrane-type metalloprotease MMP14 (refs 20, 21) in PCECs (ref. 1) leads to release of cryptic epidermal growth factor (EGF)-like ligands to stimulate the propagation of alveolar progenitor cells such as type II alveolar epithelial cells (AEC2s; refs 9, 10), initiating the regenerative alveolarization. Blockage of MMP14 activity by neutralizing antibody impeded the expansion of AECs but not PCECs, impairing neo-alveolarization and restoration of alveolar function. However, the alveologenic function of endothelial-derived MMP14 after PNX remains to be further demonstrated22,23. Moreover, the cellular and molecular basis whereby MMP14 is specifically induced in the pulmonary vascular niche1,24,25 by PNX is not defined.
Platelets are anuclear cells that constantly transit through the lung blood vessels26,27. Activated platelets not only prevent haemorrhage by forming clots, but also promote organ repair through ‘inside-out’ mobilization of trophogens and adhesion molecules28,29,30,31,32,33,34,35,36,37,38. Enriched with peptide and lipid mediators in granules39,40, platelets orchestrate multiple pathophysiological processes jointly with endothelial cells (ECs; refs 41, 42). In response to stress, platelets are activated and adhere to the vascular bed of injured organs43,44,45,46. The intimate relationship between platelets and the lung vasculature26,33 set forth the hypothesis that resection of the left lung recruits circulating platelets to prime the pulmonary vascular niche, driving alveolar regeneration.
After PNX, recruited CD41+ platelets are essential in initiating lung alveolar regeneration
To test the pulmonary recruitment of platelets by PNX, we carried out flow cytometry analysis of platelet-specific marker CD41 (also known as ITGA2B) in total cells retrieved from the remaining lobes of pneumonectomized lungs. PNX caused significant enrichment of CD41+ platelets in the lungs after PNX (Fig. 1a, b). In control mice, whereby thoracotomy was carried out without lung resection (sham), there was negligible deposition of platelets. Surface expression of P-selectin, a platelet activation marker, was detected in the majority of CD41+ platelets in the pneumonectomized lungs (Fig. 1b). Thus, activated platelets are recruited to the residual right lungs after surgical removal of the left lung lobe.
To assess the contribution of platelets to alveolar regrowth, we used Thrombopoietin-deficient (Thpo−/−) mice in which the circulating platelet number is 5% of that of wild-type (WT) littermates. In WT mice, PNX enhanced the mass, volume and respiratory function of the residual right lungs to a level comparable to those of the unresected sham lungs after 18 days (Fig. 1c–e). Notably, there was minor expansion of activated lung fibroblast cells in the residual right lungs after PNX, implicating the absence of fibrosis (Supplementary Fig. 1). By contrast, this functional regrowth of the lungs was markedly reduced in Thpo−/− mice (Fig. 1c–e). PNX-induced alveolarization is driven by the propagation of pro-surfactant protein C (SFTPC+) AEC2s (refs 9, 10) and VE-cadherin+ PCECs, which peaks at day 7 after PNX (ref. 1). Using bromodeoxyuridine (BrdU) incorporation, we found that the proliferation of AEC2s and PCECs induced by PNX was significantly lower in Thpo−/− mice than WT mice (Fig. 1f–i, Supplementary Figs1 and 2a). Reduced AEC2 proliferation in Thpo−/− mice was associated with lower restoration of aquaporin 5+podoplanin+ type I alveolar epithelial cells (AEC1s) in the pneumonectomized lungs (Fig. 1j), underscoring the potential of platelets to promote lung alveolar regeneration.
We then injected anti-CD41 monoclonal antibody (mAb) into WT mice to deplete platelets and subjected these thromobocytopenic mice to PNX (Fig. 1k and Supplementary Fig. 2). Rat IgG-treated mice were used as control. After PNX, recovery of volume, expansion of AECs and PCECs, and blood oxygenation were all inhibited in the remaining lungs of CD41 mAb-treated mice after PNX (Fig. 1l–q). Therefore, circulating platelets are essential for restoring the cellular components and respiratory function in the lungs after PNX.
Platelets deposit SDF-1 (CXCL12) to mediate PNX-induced neo-alveolarization
Activated platelet cells deploy chemokines such as stromal cell-derived factor-1 (SDF-1, also known as CXCL12) to promote organ repair24,34,40. Indeed, we found that the majority of CD41+ platelets associated with PCECs were stained as SDF-1+ in the lung cryosection after PNX but not sham operation (Fig. 2a and Supplementary Fig. 2e). This unexpected deposition of SDF-1+CD41+ platelets on VE-cadherin+ PCECs reached the highest level at day 3 and was sustained for at least 9 days after PNX (Fig. 2b, c). SDF-1 belongs to the CXC chemokine family47,48,49 and plays a key role in modulating organogenesis50,51,52,53. Indeed, SDF-1 injection significantly enhanced the recovery of AECs and restoration of gas exchange function in pneumonectomized Thpo−/− mice (Fig. 2d–f). To examine the role of SDF-1 in PNX-induced lung regrowth, we conditionally deleted the Sdf1 gene by crossing Sdf1LoxP/LoxP mice53 with tamoxifen-responsive ROSA-CreERT2 mice. Treatment of the offspring with tamoxifen led to inducible deletion of Sdf1 in adult mice (Sdf1Δ/Δ; Fig. 2g–i). Compared with control mice, Sdf1Δ/Δ mice exhibited impaired AEC2 propagation and mitigated restoration of lung weight and volume after PNX (Fig. 2j, k). Thus, SDF-1 signalling is critical for lung alveolar regeneration induced by PNX.
We then formally deciphered the functional contribution of SDF-1 from various niche cell components to lung regeneration (Supplementary Fig. 3). The in vivo role of platelet-derived SDF-1 was examined by breeding a Platelet factor 4-Cre mouse line with floxed Sdf1 mice (Fig. 3a). This strategy specifically deletes Sdf1 from mouse platelets and platelet progenitors (Sdf1ΔPLT/ΔPLT). Compared with control Sdf1ΔPLT/+ mice, the restoration of lung mass and volume and regeneration of AECs and PCECs were significantly reduced in Sdf1ΔPLT/ΔPLT mice (Fig. 3b–j). By contrast, deletion of Sdf1 from both myeloid and endothelial cells caused an insignificant reduction in tested lung regenerative responses (Supplementary Fig. 3). Notably, Sdf1ΔPLT/ΔPLT mice did not exhibit altered production of growth factors from platelets, recruitment of haematopoietic cells or pulmonary fibrin deposition after lung injury (Supplementary Fig. 4). Hence, deployment of SDF-1 from activated platelets is indispensable to elicit regenerative alveolarization after PNX (Fig. 3k).
Intravascular infusion of SDF-1+ platelets rescues impaired alveolar regeneration in Thpo−/− mice
The importance of platelets in evoking lung regeneration led us to postulate that infusion of platelets into Thpo−/− mice would rescue the defective regeneration. To this end, we adopted a platelet-infusion model27 to intravenously transfuse platelets into mice (Fig. 4a). After jugular vein infusion, platelets preferentially accumulated in the lungs of Thpo−/− mice after PNX (Fig. 4b, c). As such, this platelet-infusion strategy allows for a ‘gain of function’ study to interrogate the influence of platelets on lung alveolar regeneration.
We then compared the effects of Sdf1+/+ and Sdf1-deficient (Sdf1−/−) platelets on the impaired lung regeneration in Thpo−/− mice (Fig. 4d). Sdf1−/− and Sdf1+/+ platelets were isolated from Sdf1Δ/Δ and WT littermates and infused into pneumonectomized Thpo−/− mice (Supplementary Fig. 5). Whereas after PNX similar numbers of infused platelets adhered to PCECs, infusion of Sdf1+/+ but not Sdf1−/− platelets into Thpo−/− mice rescued the restoration of lung mass and volume (Fig. 4e, f) and pulmonary function (Fig. 4g), as well as expansion of AECs and PCECs (Fig. 4h–l). Consequently, Sdf1+/+ platelet infusion to Thpo−/− mice induced significantly elevated neo-alveologenesis when compared with Sdf1−/− platelet transplantation, as shown by higher alveolar number and lower mean linear intercept (Fig. 4m, n, o). Thus, increased bioavailability of SDF-1 from the infused platelets rescued the defective neo-alveolarization in pneumonectomized Thpo−/− mice.
Platelet-derived SDF-1 induces membrane MMP14 in PCECs to release EGF-like ligand, stimulating neo-alveologenesis
Induction of membrane MMP14 in PCECs by PNX causes release of EGF-like ligands such as heparin-binding EGF-like growth factor (HB-EGF; ref. 22) to stimulate the propagation of adjacent AEC2s. Close association between PCECs and recruited platelets after PNX suggests that platelets stimulate alveolar regeneration by priming the pro-regenerative function of the PCEC niche1,15,16,25,54. Indeed, PCEC expression of MMP14 in Sdf1ΔPLT/ΔPLT mice was significantly lower than that in control mice after PNX (Fig. 5a). Thus, we analysed how platelet SDF-1 regulates PCEC deployment of MMP14. The supernatant of ADP-stimulated Sdf1+/+ but not Sdf1−/− platelets caused membrane MMP14 upregulation in PCECs, which was blunted by the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 (Fig. 5b, c). MMP14 was similarly upregulated in both mouse and human PCECs after stimulation of recombinant SDF-1, and this upregulation was abrogated by genetic silencing of SDF-1 receptors Cxcr4 and Cxcr7 (also known as Ackr3) in PCECs (Fig. 5d–f). These data imply that platelets release SDF-1 to induce pro-regenerative MMP14 in the PCEC niche, igniting regeneration without causing fibrosis (Supplementary Fig. 5a).
Blocking induction of endothelial MMP14 with PI3K inhibitor raised the hypothesis that, after PNX, platelets deploy SDF-1 to stimulate Akt-dependent MMP14 upregulation. To test this notion, we measured the Akt activation/phosphorylation in PCECs of pneumonectomized WT and Thpo−/− mice (Fig. 5g). PNX caused a time-dependent Akt phosphorylation in the PCECs of WT mice. By contrast, this PCEC Akt activation by PNX was significantly lower in Thpo−/− mice, which was enhanced by infusion of Sdf1+/+ rather than Sdf1−/− platelets (Fig. 5h, i). These findings suggest that deposition of SDF-1 by platelets stimulates Akt-dependent upregulation of MMP14 in PCECs after PNX.
Cellular surface localization of MMP14 is critical for the protease activity of membrane-type MMP14 (refs 20, 22, 23). Treatment of PCECs with supernatant from activated platelets stimulated SDF-1-dependent membrane localization of MMP14 protein (Fig. 5j). To determine the distribution of MMP14 in regenerating PCECs, we used an in situ pulmonary vascular perfusion system to selectively biotinylate and fractionate PCEC membrane proteins (Fig. 5k and Supplementary Fig. 6a)55. At day 7 after PNX, the MMP14 level in the isolated PCEC membrane proteins was significantly higher in the pneumonectomized lungs of WT but not Thpo−/− mice (Fig. 5l). Both thrombopoietin (TPO) injection and transplantation of WT platelets to Thpo−/− mice augmented membrane enrichment of MMP14 in PCECs (Fig. 5m). Subsequently, release of an epithelially active substrate of MMP14, HB-EGF, in the alveolar space was augmented in Thpo−/− mice transplanted with Sdf1+/+ but not Sdf1−/− platelets, as shown by an elevated HB-EGF protein level in bronchioalveolar lavage fluid (BALF; Fig. 5n–p). These findings indicate that SDF-1 from activated platelets primes the PCEC niche to deploy membrane MMP14, initiating propagation of adjacent AECs.
Inducible endothelial-specific deletion of Mmp14 in mice blocked neo-alveologenesis after PNX
We then used an EC-specific genetic deletion strategy to assess the contribution of endothelial-derived MMP14 in stimulating neo-alveologenesis. Tamoxifen-inducible EC-specific mouse deleter VE-Cad-CreERT2/Cdh5(PAC)-CreERT2 mice56 were bred with mice harbouring LoxP-site-flanked Mmp14 (ref. 21). Intraperitoneal injection of tamoxifen into resultant offspring induced EC-specific Mmp14 ablation (Mmp14iΔEC/iΔEC) in adult mice (Fig. 6a and Supplementary Fig. 6c, d). After PNX, restoration of lung mass and volume and cellular propagation were markedly reduced in Mmp14iΔEC/iΔEC, compared with control Mmp14iΔEC/+, mice (Fig. 6b–f). Notably, infusion of platelets failed to rescue the impaired functional alveolar regrowth in Mmp14iΔEC/iΔEC mice after PNX (Fig. 6g–j). By contrast, injection of MMP14 downstream effector recombinant EGF into Thpo−/− mice rescued the defective lung regeneration after PNX (Fig. 6k–o and Supplementary Fig. 6e, f). These data suggest that recruited platelets after PNX deploy MMP14 in the PCEC niche to stimulate EGF receptor (EGFR) signalling in proximal AEC2s and instigate their expansion, driving neo-alveologenesis and restoration of pulmonary function (Fig. 6p).
SDF-1 receptors CXCR4 and CXCR7 are required for induction of alveologenic MMP14 in PCECs
To examine the molecular mechanism whereby platelet SDF-1 activates the PCEC niche, we tested the contribution of SDF-1 receptors CXCR4 and CXCR7 on PCECs after PNX. Injection of CXCR4 antagonist AMD3100 blocked cell proliferation and lung function recovery following PNX (Supplementary Fig. 7), suggesting the importance of CXCR4 in promoting PNX-induced alveolar regrowth. To circumvent the vascular defect and embryonic lethality caused by Cxcr4 genetic deletion48, we inducibly deleted Cxcr4 and Cxcr7 from the ECs of adult mice (Cxcr4iΔEC/iΔEC and Cxcr4iΔEC/iΔECCxcr7iΔEC/iΔEC; Fig. 7a and Supplementary Fig. 8a, b). Alveolar regeneration in Cxcr4iΔEC/iΔEC mice was significantly impaired when compared with control Cxcr4iΔEC/+ mice, including expansion of AECs and PCECs (Fig. 7b–e), recovery of lung function (Fig. 7f, g), MMP14 induction in PCECs (Fig. 7h) and restoration of mass and volume (Fig. 7i, j). Of note, this defect in functional alveolar regrowth was further exacerbated in Cxcr4iΔEC/iΔECCxcr7iΔEC/iΔEC mice. Impaired lung regeneration in Cxcr4iΔEC/iΔEC and Cxcr4iΔEC/iΔECCxcr7iΔEC/iΔEC mice was associated with attenuation of Akt activation in PCECs (Fig. 7k and Supplementary Fig. 8c). To illustrate the influence of platelets on SDF-1-mediated signalling in the PCEC niche, we infused platelets into Cxcr4iΔEC/iΔEC and Cxcr4iΔEC/iΔECCxcr7iΔEC/iΔEC mice and tested the regenerative response (Fig. 7l). Infusion of SDF-1+ platelets into mice did not restore the alveolar release of HB-EGF in either Cxcr4iΔEC/iΔEC or Cxcr4iΔEC/iΔECCxcr7iΔEC/iΔEC mice (Fig. 7m, n). Taken together, these findings suggest that platelets deploy SDF-1 to trigger CXCR4 and CXCR7 signalling in PCECs, enabling a pro-regenerative niche that drives regenerative alveolarization after PNX.
Modulation of SDF-1 signalling in the pulmonary vascular niche by the VEGFR2/FGFR1 pathways
Activation of PCECs during alveolarization is a coordinated process that requires synergistic effects of various signalling molecules, including the angiogenic factors vascular endothelial growth factor-A (VEGF-A) and fibroblast growth factors (FGFs; ref. 1). Indeed, activation of both VEGF receptor 2 (VEGFR2) and FGF receptor 1 (FGFR1) pathways was attenuated in PCECs of Thpo−/− mice after PNX (Fig. 8a–c). This finding implies that activation of VEGFR2 and FGFR1 in PCECs after PNX is at least partially dependent on platelets. To test the correlation of SDF-1 signalling with the VEGFR2/FGFR1 pathways, we tested the expression of CXCR4 in mice with EC-specific deletion of VEGFR2 and FGFR1 (ref. 1; Vegfr2iΔEC/iΔECFgfr1iΔEC/+) after PNX (Fig. 8d, e). Notably, the protein level of CXCR4 in PCECs was significantly upregulated by PNX in control but not Vegfr2iΔEC/iΔECFgfr1iΔEC/+ mice. These data suggest that SDF-1 signalling (receptor CXCR4) in PCECs is regulated by the previously shown pro-regenerative VEGFR2 and FGFR1 pathways after PNX.
We then used Vegfr2iΔEC/iΔECFgfr1iΔEC/+ mice and Cxcr4iΔEC/iΔECCxcr7iΔEC/iΔEC mice to reveal the functional regulation of SDF-1 signalling by VEGFR2/FGFR1 in the PCEC niche after PNX (Fig. 8f). First, restoration of lung function (blood oxygenation) was significantly improved in Vegfr2iΔEC/iΔECFgfr1iΔEC/+ mice by SDF-1 injection after PNX. By contrast, VEGF injection into Cxcr4iΔEC/iΔECCxcr7iΔEC/iΔEC mice failed to enhance the recovery of lung function in mice (Fig. 8g). The different effects caused by VEGF-A and SDF-1 injection implicate that CXCR4/7-mediated SDF-1 signalling in PCECs is downstream of the VEGFR2 pathway.
This potentiation of SDF-1 signalling by the VEGFR2/FGFR1 pathways was further demonstrated by the analysis of Akt activation in PCECs (Fig. 8h, i). Compared with WT mice, Akt activation in PCECs was blocked in both Vegfr2iΔEC/iΔECFgfr1iΔEC/+ and Cxcr4iΔEC/iΔECCxcr7iΔEC/iΔEC mice. Of note, whereas SDF-1 injection into Vegfr2iΔEC/iΔECFgfr1iΔEC/+ mice restored PCEC Akt activation, Akt activation in PCECs of Cxcr4iΔEC/iΔECCxcr7iΔEC/iΔEC mice was not enhanced by VEGF-A injection. Thus, activation of VEGFR2 and FGFR1 promotes SDF-1–CXCR4/7 signalling to stimulate activation of the PCEC niche, driving the regenerative alveolarization process (Fig. 8j), and it is plausible that, after PNX, platelet-derived SDF-1 cooperates with other pathways to synergistically prime pro-regenerative PCECs, initiating alveolar regeneration (Fig. 8k)29,33,34.
Here, the centrality of platelet-derived SDF-1 in inducing alveolar regeneration was revealed by ‘platelet-specific’ gain and loss of function studies: first, regenerative alveolarization was impaired in thrombocytopenic mice caused by TPO knockout (Thpo−/−) and pharmacological platelet depletion (Fig. 1); second, platelet-specific and inducible genetic ablation of the Sdf1 gene abrogated lung alveolar regeneration (Figs 2 and 3); third, intravascular transfusion of Sdf1+/+ platelets, but not Sdf1−/− platelets, rescued the defective alveolarization of Thpo−/− mice (Figs 4 and 5); last, using an EC-specific gene deletion strategy (Mmp14iΔEC/iΔEC and Cxcr4iΔEC/iΔECCxcr7iΔEC/iΔEC), we demonstrate that this rescue effect of SDF-1 platelets is mediated through CXCR4/7-dependent deployment of the pro-regenerative MMP14 pathway in the PCEC membrane (Figs 6–8). Hence, we propose that PNX recruits SDF-1+ platelets to prime PCECs in the remaining lungs, constituting a haemo-vascular niche to orchestrate functional restoration of pulmonary tissues.
How the loss of the left lung recruits and activates platelets in the right lung remains to be studied. It is plausible that, after PNX, increased blood flow and circumferential shear force in the right lung activate platelets to adhere to pulmonary capillaries31,32,35,36,57. Alternatively, perturbation of biomechanical forces within the remaining lung activates pulmonary capillaries, thereby recruiting and activating circulating platelets42. In addition, how activated platelets influence other types of lung progenitor cell needs to be investigated in the future11,12,13,14,58.
Of note, the morphology of AEC1s is compromised when regeneration fails. After PNX, loss of the left lung lobe caused significantly increased mechanical stretch in the remaining lungs, possibly stimulating the matrix remodelling process6. Conceivably, in WT mice with normal regenerative capacity, this remodelling process coordinately accompanies the expansion of AEC1s. By contrast, when regeneration/cellular propagation fails in platelet-depleted mice or mice with a defective vascular niche, the alveolus undergoes remodelling in the absence of concomitant expansion of AECs. As such, in these mutant or antibody-treated mice, discordance between alveolar remodelling and lack of concurrent AEC propagation not only prevented the growth of new alveoli but also disrupted the morphology of existent AECs, compromising alveolar architecture.
Here we have used cell-type-specific ‘loss and gain of function’ studies to demonstrate that recruited platelets release SDF-1 to activate CXCR4/CXCR7 on PCECs in the early phase after PNX (days 1–5), eliciting cellular propagation and alveolarization that peaks at day 7. It is likely that platelets represent a subpopulation of bone-marrow-derived cells recruited into the lungs of pneumonectomized mice49. Whether bone-marrow-derived cells facilitate the pulmonary recruitment of activated platelets after PNX remains to be investigated. Furthermore, SDF-1 from other niche cells50,51,52,53 could play a prominent role in modulating tissue repair in other injury models.
We have also reconciled the mechanistic link between the previously demonstrated VEGFR2/FGFR1 pathways1,59 and the presented endothelial SDF-1–CXCR4/7 interaction24 in the study. SDF-1–CXCR4 signalling is regulated by the VEGFR2/FGFR1 pathways in PCECs after PNX, and Thpo−/− mice also exhibited blocked VEGFR2/FGFR1 signalling in PCECs. These findings imply that platelets also contribute to activation of the VEGFR2/FGFR1 pathways, which further potentiates SDF-1 signalling in PCECs. It is likely that activation of both pathways in PCECs leads to MMP14-dependent lung alveolarization, which is initially stimulated by platelets after PNX.
Lung disease remains the worldwide leading cause of death for adults6,7,15,60,61,62,63. Damage to the lungs after trauma or chemical injury results in initiation of reparative pathways. However, in most instances lung repair is insufficient to overcome the loss of functional parenchymal tissues13,64,65,66. Our finding implies that restoration of functional lung mass could be elicited in adult mammals by a coordinated interaction between alveolar progenitor cells and pro-regenerative cues from the vascular niche. As such, properly stimulating the cellular crosstalk between alveolar progenitor cells with the surrounding niche5,9,10,11,12,13,14,67,68 could instigate regeneration in adult human lungs with limited regenerative capacity, implicating the translational value of our study in combating chronic destructive lung diseases.
Uncovering the effects of platelet-derived SDF-1 in inducing lung regeneration holds further clinical implications. First, it is conceivable that thrombocytopenic patients will exhibit impaired regenerative ability after lung injury and show poor prognosis. To this end, periodic administration of SDF-1 or EGF to thrombocytopenic patients (for example, in an intensive care unit) could stimulate functional lung repair. Furthermore, the finding that infusion of SDF-1+ platelets initiates and sustains functional alveolarization suggests that transplantation of properly primed platelets represents an effective cell therapy approach to enable lung regeneration and repair.
Taken together, we have revealed a paradigm that loss of the left lung lobe recruits platelets to deploy SDF-1, preconditioning PCECs and eliciting lung alveolar regeneration. Infusion of functional platelets rescued the impaired lung regeneration by re-establishing a pro-regenerative haemo-vascular niche. Unravelling the cellular and molecular basis whereby platelets orchestrate lung regeneration permits a clinical strategy to initiate regenerative approach for lung diseases.
The Cdh5(PAC)-CreERT2 (VE-cadherin-CreERT2) mouse line was provided by R. Adams56, Rosa-CreERT2 animals expressing tamoxifen-responsive inducible CreERT2, loxP-site-flanked Cxcr4 and Cxcr7 mice and Thpo−/− mice have been described previously1,25,34. Floxed Mmp14 mice were offered by S. Weiss at the University of Michigan21. C57/B6, Pf4-Cre, floxed Sdf1 (ref. 53) and actin-tdTomato mice ubiquitously expressing tdTomato fluorescent protein (β-actin promoter-driven tdTomato) were obtained from Jackson Laboratory. To activate Cdh5(PAC)-CreERT2 and Rosa-CreERT2, mice were treated with tamoxifen six times at dose of 150 mg kg−1. After daily injection of tamoxifen for three consecutive days, mice were rested for three days and then treated with tamoxifen for another three days. Mice were used for surgery or analysis at least 30 days after final tamoxifen treatment. To compare phenotypes between different genotypes, age/sex/weight-matched littermates were used in corresponding experimental groups. Mice at 20–28 g bodyweight were used. Investigators who established the mouse lung regeneration model and analysed the pattern and extent of cell activation/proliferation were randomly assigned with animals or samples from different experimental groups and were blind to the genotypes of individual groups. All animal procedures were under the guidance of the NIH and approved by IACUC at Weill Cornell. Chemicals were from Sigma unless otherwise specified.
Left lung unilateral pneumonectomy model and measurement of alveolar regeneration.
The PNX procedure was adapted as described1. Mice were anaesthetized with 100 mg kg−1 intraperitoneal ketamine and 10 mg kg−1 xylazine. Orotracheal intubation was carried out in anaesthetized and mechanically ventilated mice. The left lung lobe was resected with a suture tied around the hilum. Sham-operated mice were subjected to thoracotomy without lobe resection. To determine the role of recombinant EGF in alveolar regeneration, mice were injected with 500 μg kg−1 recombinant mouse EGF (Abcam) on a daily basis after PNX.
Immunostaining and morphometric analysis.
Each parameter from each individual animal was measured at least twice and averaged. Before killing, mice were anaesthetized, blood was obtained through the inferior vena cava and the remaining right lung lobes received an intratracheal instillation of reconstituted optimal cutting temperature compound (Tissue-Tek) to a pressure of 25 cm H2O. The trachea was then tied under pressure, and the lungs were cryoprotected and snap frozen in optimal cutting temperature compound. For immunofluorescence microscopy, the tissue sections (10 μm) were blocked with 5% normal donkey serum/1% bovine serum albumin/0.1% Triton X-100 and incubated in primary antibodies. PCECs were identified by staining of anti-VE-cadherin polyclonal antibodies (R&D Systems AF1002, 2 μg ml−1); AEC1s and AEC2s were determined by staining with podoplanin (R&D Systems, AF3244, 2 μg ml−1) and aquaporin 5 (Abcam, ab78486, 2 μg ml−1) for AEC1s and SFTPC (Millipore, Ab3786, 2 μg ml−1) for AEC2s. Platelet deposition was detected using anti-CD41 mAb (MWReg30, BD Biosciences, 1 μg ml−1). After incubation in fluorophore-conjugated secondary antibodies (2.5 μg ml−1, Jackson ImmunoResearch), nuclear counterstaining was carried out with DAPI (Invitrogen). No appreciable staining was observed in isotype IgG controls. AEC2s and PCECs were quantified by staining with antibodies recognizing SFTPC and VE-cadherin, respectively. Fluorescent images were captured on an AxioVert LSM710 microscope (Zeiss). For image analysis, staining signals in each slide (such as SFTPC, VE-cadherin, podoplanin, aquaporin 5 and CD41) were independently evaluated and quantified by two investigators from five randomly selected fields of view. Analysis of the image was carried out blind. Investigators were not aware of the genotype of animals or identity of samples during scoring.
Lung cell proliferation in vivo was measured by BrdU uptake. Mice received a single intraperitoneal injection of BrdU (Sigma; at a dose of 50 mg per kg animal weight) 60 min before killing, and the incorporation of BrdU was measured by immunostaining on cryosections and flow cytometry as previously described1,51. Cryosections were stained using the BrdU Detection System (BD Biosciences) and fluorophore-conjugated secondary antibodies (2.5 μg ml−1, Jackson ImmunoResearch)1,51. The extent of BrdU incorporation was first determined in sham-operated mice of all used genotypes. To assess the difference in cell proliferation after PNX, the percentage of BrdU+VE-cadherin+ proliferating PCECs and BrdU+SFTPC+ AEC2s in both control and mutant groups were compared at day 7.
Measurement of functional alveologenesis after PNX.
Lung respiratory function parameters, including inspiratory capacity and lung parenchymal tissue compliance, were measured using the forced oscillation technique operated by flexiWare 7 software in a computer-controlled piston ventilator (SCIREQ). Inspiratory capacity was measured during a recruitment manoeuvre using the above software. Oxygen tension in the arterial blood was measured as previously described using I-Stat69 (Abbott Laboratories).
H&E staining was carried out on retrieved lung tissues to evaluate alveologenesis after PNX. Alveolar structure in each H&E slide was independently quantified by two investigators from five random fields. Mean linear interception (Lm) was measured as previously described70. Briefly, 10 equally distributed horizontal lines and 11 evenly distributed vertical lines were drawn over an H&E-stained section. For each line, the intercepts with the alveolar tissue are counted. Lm is calculated as the averaged ratio between line length and the number of intercepts placed on the lung section.
Alveolar number was determined as previously described8. Briefly, the lungs were cut into 3 mm thick lung slices and subsequent cutting of 3 mm wide bars with randomized tissue orientation. Embedded bars were cut into 20 μm thick serial sections. Sections were collected on slides and subjected to H&E staining for determination of the mean bar thickness. The block was then cut to a series of 5 μm thick serial sections and collected on slides. The ratio of serial section thickness/mean bar thickness was then determined. To obtain a slide for alveolar number determination, a series of sections for each tissue block was systematically scanned along the x- and y-axes to yield a uniform, random sample with disector pairs of 600 μm × 600 μm sections. Then the number of alveoli was measured by assessing the Euler number (x) of the network of alveolar openings within a counting frame. For measurement of the Euler number, all alveolar openings in the examined section were counted in each frame. To increase the efficiency of alveolar number measurement, both directions in the frame were measured in each individual field of view. Then the total number of alveoli per lung was calculated based on alveolar number within the area of the counting frame and the disector pair size on the slide, as well as the ratio between serial section thickness and mean bar thickness.
Isolation and analysis of mouse PCECs.
Isolation of PCECs and examination of phosphorylation and total protein levels of Akt, VEGFR2, FRS2, CXCR4 and MMP14 were carried out as described1,51. Briefly, the lungs was perfused at 5 ml min−1 through the pulmonary artery with Liver Perfusion Medium (Invitrogen) at 37 °C for 10 min, followed by perfusion with Liver Digest Medium (Invitrogen) for another 10 min. 1 ml digestion medium was also injected into the alveolar space through the exposed trachea. The lung tissue was then dissociated in Hepatocyte Wash medium (Invitrogen) and passed through Dacron fabric with 70 μm pores, and the flowthrough containing PCECs was collected, washed twice and pelleted at 350g for 7 min. Fractions containing PCECs were enriched, washed with an equal volume of PBS and centrifuged at 900g for 7 min. The cell pellet was then washed with PBS at 350g for 7 min and incubated with Dynabeads magnetic beads (Invitrogen) coated with rat anti-mouse CD31 and intercellular cell adhesion molecule (ICAM)-2 antibodies (MEC13.3, BD Biosciences). The purification of CD31+ICAM2+ PCECs was carried out following the manufacturer’s protocol. To maintain protein phosphorylation in the isolated PCECs, mouse lungs were perfused through the pulmonary artery with phosphatase inhibitor cocktails (Sigma) after mice were killed, and whole processing was carried out in the presence of phosphatase inhibitor cocktails.
Isolated platelets or cultured PCECs were lysed with RIPA (radio-immunoprecipitation assay) buffer: 1× tris-buffered saline, 1% (v/v) Nonidet P-40, 0.5% (w/v) sodium deoxycholate, 0.1% (v/v) SDS, 1 mM sodium orthovanadate, 10 mM NaF and 1× protease inhibitor cocktail (Roche). Phosphorylation and protein levels of VEGFR2 and the FGFR1 downstream effector FRS2 in PCECs were determined as described51.
Equal amounts of protein were subjected to SDS–polyacrylamide gel electrophoresis in 4–12% Bis-Tris gels (Invitrogen). Proteins were transferred to nitrocellulose membranes, and underwent immunoblotting with antibodies against VE-cadherin (R&D, AF1002, 1 μg ml−1), VEGFR2 (Cell Signaling, 2472, 1 μg ml−1), phosphorylated VEGFR2 (Cell Signaling, 3817, 1 μg ml−1), total AKT (Cell Signaling, 9272, 1 μg ml−1), phosphorylated Akt (Cell Signaling, 2965, 1 μg ml−1), total FRS2 (Abcam, ab137458, 1 μg ml−1), total CXCR4 (Abcam, ab2074, 2 μg ml−1), total MMP14 (Abcam, ab51074 and ab53712, 1 μg ml−1) and SDF-1 (Cell Signaling, 3740, 2 μg ml−1). β-actin (Santa Cruz, sc-1616, 2 μg ml−1) in the loaded sample was also tested to control for the loaded protein amount.
To test the protein components in the alveolar space, BALF was obtained from sham or pneumonectomized mice by lavaging 1 ml of PBS through the trachea, and retrieved BALF was concentrated to 100 μl with a protein concentration column (Millipore). The HB-EGF protein level in BALF was assessed as previously described1, using rabbit polyclonal antibody from Santa Cruz.
Depletion of circulating platelets; stimulation of thrombopoiesis.
To stimulate thrombopoiesis, recombinant TPO was intraperitoneally injected into Thpo−/− or WT mice at a dose of 25 μg kg−1 on a daily basis 10 days before PNX and afterwards. To deplete circulating platelets, rat anti-mouse CD41 mAb (BD Biosciences, clone MWReg30) was intraperitoneally injected into mice at a dose of 10 mg kg−1 every 3 days. Recombinant TPO, EGF and SDF-1 were obtained from PeproTech. Vehicle for individual cytokines was also injected as a control group.
The effects of injected cytokine on alveolar regeneration were evaluated with control groups treated with vehicle, including alteration in circulating platelets and parameters of alveologenesis (as described above). In particular, mouse platelets were collected at indicated times from the retrobulbar venous plexus of anaesthetized mice, using heparinized haematocrit tubing. The platelet count in peripheral blood was then determined with an automated Advia 120 Hematology System (Siemens Healthcare).
Isolation and stimulation of mouse platelets and intravascular infusion of platelets into mice.
To maximize the yield of platelets, the blood remaining from this first centrifugation was diluted with PBS to the original volume and recentrifuged. Mouse platelets isolated by centrifugation were treated with agonist ADP (5 or 10 μM) or 0.1 U ml−1 thrombin. After agonist addition, the reaction was terminated and incubation microfuge tubes were centrifuged (1 min, 14,000g). The supernatant of the stimulated platelets was then aliquoted for activation of primary PCECs.
The strategy to infuse platelets through the jugular vein was adopted from previous work49. Platelets were isolated and concentrated from mice with an inducible deletion of Sdf1 (Sdf1Δ/Δ mice) to obtain Sdf1−/− platelets. Platelets from their WT littermates, or β-actin promoter-driven tdTomato reporter mice, were used as Sdf1+/+ platelets. After isolation, 2 × 109 Sdf1+/+ or Sdf1−/− platelets were infused through the exposed jugular vein of mice over a period of 15 min. This injection route yielded the highest accumulation of platelets in the pulmonary vasculature of recipient mice. To track the localization of infused CD41+tdTomato+ platelets in the recipient mouse lungs, total cells were isolated from the recipient and analysed for surface expression of tdTomato fluorescent signal and CD41. Immunofluorescent staining of CD41 and VE-cadherin was also carried out to examine the association of PCECs with transplanted platelets, as described in ‘Immunostaining and morphometric analysis’.
Cultivation of mouse and human PCECs and gene knockdown.
Primary mouse PCECs were obtained from Angiocrine Bioscience (New York) and cultured as previously described1. Human PCECs were from ScienCell Research Laboratories and cultivated following the vendor’s instructions. To knock down Cxcr4 and Cxcr7 selectively in PSECs, Cxcr4 and Cxcr7/scrambled shRNA lentiviruses were generated by co-transfecting 15 μg of shuttle lentiviral vector containing Id1/scrambled shRNA, 3 μg pENV/VSV-G, 5 μg pRRE and 2.5 μg pRSV-REV in 293T cells using FuGENE 6 (Roche Applied Science). Viral supernatants were concentrated by ultracentrifugation. These concentrated viral preparations were used to transduce PCECs. Human CXCR4 was silenced by treatment with combined shRNA clones: TRCN0000004052, TRCN0000004053, TRCN0000004054 and TRCN0000004055 (Open Biosystems), and CXCR7 was knocked down with clone TL305345 (OriGene). Cxcr4 was knocked down in mouse PCECs by transduction with a combination of clones TRCN0000028678, TRCN0000028704 and TRCN0000028724. Cxcr7 was silenced in mouse PCECs with shRNA cloneTL515747 from Origene.
Supernatant from platelets was used to treat PCECs after starvation in minimal medium that consists of Medium 199 (HyClone) and 2% BSA. Six hours after treatment with platelet supernatant or 10 ng ml−1 SDF-1, PCECs were retrieved for analysis of MMP14 protein level by immunoblot after cell lysis, immunostaining and flow cytometry. MMP14 was detected by rabbit polyclonal antibody from Abcam (ab51074 and ab53712, 1 μg ml−1).
Isolation of membrane protein of PCECs after pulmonary perfusion and in situ biotinylation.
Biotinylation of PCEC membrane protein in mice was achieved by pulmonary perfusion through the pulmonary artery53. After the mouse pulmonary artery was exposed, a 25-gauge catheter was inserted and fastened. PBS was used to perfuse the mouse pulmonary vasculature. Then the pulmonary vasculature was perfused with PBS containing 10 mg ml−1 membrane-impermeable sulpho-NHS-LC-biotin that recognizes primary amine in protein (Pierce) at 1 ml min−1 for 10 min. PCEC membrane proteins that are accessible to circulation were biotinylated. After unconjugated biotin was quenched by 20 mg glycine, lung tissues were harvested and homogenized, and biotinylated proteins were isolated using 500 μl g−1 tissue streptavidin beads (Pierce) and subjected to immunoblot against MMP14 and VE-cadherin after sonication and boiling. This in situ biotinylation strategy provides an approach to isolate and assay membrane fractions of lung ECs. The protein level of MMP14 in isolated membrane proteins after PNX was determined by immunoblot (Abcam), and the EC-specific marker VE-cadherin was also blotted (R&D systems) as a control for the loading of endothelial membrane proteins. To control for the purity of isolated EC membrane proteins, endothelial membrane proteins such as platelet-endothelial cell adhesion molecule (PECAM)-1 (Santa Cruz Biotechnology, sc-1506, 10 μg ml−1), ICAM-1 (Santa Cruz Biotechnology, sc-1511, 10 μg ml−1), VEGFR2, angiotensin converting enzyme (sc-20998, 2 μg ml−1), integrin αv (sc-6618, 5 μg ml−1), α5 (sc-10729, 5 μg ml−1), E-cadherin (sc-7870, 10 μg ml−1) and cytoplasmic proteins α-tubulin (sc-53646, 10 μg ml−1) and β-actin were also examined in the isolated membrane fractions by immunoblot as described above.
Mouse model of unilateral in situ lung ischaemia–reperfusion.
Acute lung injury was also introduced by a mouse model of lung ischaemia–reperfusion (I/R). Unilateral left lung I/R was created using a protocol previously described in detail69. Briefly, after carrying out a thoracotomy in ventilated anaesthetized mice, the hilum of the left lung was cross-clamped for 30 min followed by 120 min of reperfusion. Five minutes before killing, 400 U of heparin was injected intravenously to prevent post-mortem clotting and the left lobe of the lung was excised. Sham-operated mice underwent the same procedure except that clamping of the hilum was omitted. Lung specimens were weighed and homogenized in PBS (pH 7.2) containing protease inhibitor cocktail (Sigma), aprotinin (30 KIU ml−1), heparin 15 U ml−1 and 100 mM 6-aminohexanoic acid. Aliquots of tissue homogenates were prepared for analysis. Lung fibrin was extracted from one aliquot and detected by western blot using fibrin β-chain-specific antibody 350 (American Diagnostica, 5 μg ml−1), as described previously69. Total lung proteins were extracted with 1% SDS and 1% Triton X-100 and subjected to western blot using anti-β-actin. Lung fibrin was quantified by densitometric analysis and normalized to β-actin. Lung fibrin deposition in lungs was compared between different groups.
Flow cytometric analysis.
To reveal platelet and PCEC activation, deposition and proliferation, total lung cells were isolated from perfused mouse lung tissues after dissociation with medium containing collagenase/dispase/DNase1. Retrieved lung cells were incubated with conjugated antibodies and analysed by flow cytometry on LSRII-SORP (BD; ref. 51). Data were processed with FACSDiva 6.1 software (BD). All doublets were ruled out by FSC-W × FSC-H and SSC-W × SSC-H analysis. Monoclonal antibodies were conjugated to Alexa Fluor dyes or Qdots according to manufacturers’ protocols (Molecular Probes/Invitrogen), including VE-cadherin (clone BV13, ImClone, 5 μg ml−1), CD41 (clone MWReg30, BD Bioscience, 5 μg ml−1) and P-selectin (clone RB40.34, BD Bioscience, 5 μg ml−1). Single-stained channels were used for compensation. Flow cytometry analysis was carried out using various controls, including unstained cells, isotype antibodies and fluorophore minus one controls for determining gates and compensations in flow cytometry.
Experiments were repeated at least three times. We included all tested animals for quantification to analyse the statistical difference. A representative image from each animal group is presented in the figure. If higher variability was found, we increased the sample size to fully confirm statistical significance. No statistical method was used to predetermine sample size. Statistical analysis between individual experimental groups was determined by an unpaired two-tailed t-test. All data points from an individual animal or cell are presented in the format of a dot plot.
Reproducibility of experiments.
All presented representative images were obtained from independently repeated experiments. Immunostaining images in Figs 1f, j, o, 2a, e, j, 3g, j, 4h, l, 5a, i, 6d, k, m, o and 7b and Supplementary Figs 1a–d, 2a, c, e, 3g, h, 5a and 7a were independently repeated three times. Immunoblot images in Figs 2i, 3b, 5b, d, e, g, h, l, m, n, 7k, m and 8b, e, h and Supplementary Figs 6a, c and 8a, b were from four repeated experiments, and flow cytometry micrographs in Figs 1b, h, n, 2b, 3h, 4i, 6f, n and 7e, h and Supplementary Fig. 7c were repeated three times. Used animal numbers per groups are described in the respective figure legends.
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We are grateful to T. Hla, R. Nachman and A. Choi (Weill Cornell) and S. Albelda and D. Cines (University of Pennsylvania) for critically evaluating our manuscript. Thpo−/− mice were provided by F. de Sauvage at Genentech. We would also like to thank R. H. Adams, S. J. Weiss and Y-R. Zou for offering inducible EC-specific Cdh5-(PAC)-CreERT2 and floxed Mmp14 and Cxcr4 mouse lines. We are indebted to M. E. Penfold at Chemocentryx for his help in providing floxed Cxcr7 mice. B-S.D. is supported by a National Scientist Development Grant from the American Heart Association (number 12SDG1213004). B-S.D. and Z.C. are both supported by Druckenmiller Fellowships from the New York Stem Cell Foundation. S.Y.R. is supported by Biotime. S.R. and K.S. are supported by Ansary Stem Cell Institute, the Empire State Stem Cell Board and New York State Department of Health grants (C026878, C028117, C029156), and S.R. is supported by the National Heart, Lung, and Blood Institute R01HL097797, R01HL115128, R01HL119872 and R01HL128158, National Cancer Institute U54CA163167 and the National Institute of Diabetes and Digestive and Kidney Diseases R01DK095039.
The authors declare no competing financial interests.
Integrated supplementary information
Supplementary Figure 1 Characterization of the lungs of indicated mouse genotypes after left lung pneumonectomy (PNX) and sham-operation.
(a,b) Protein levels of fibroblast cell markers desmin (a) and collagen (b) were tested by immunostaining and sirius red staining at indicated time points after PNX. Fibrotic mouse lungs at day 21 after intratracheal injection of Bleomycin (I.T. Bleo) was used as positive control. Scale bar = 50 μm. (c,d) Sham operation was performed by thoracotomy without resection of left lung tissues. To introduce inducible or cell type-specific deletion of Sdf1, floxed Sdf1 mice were crossed with mice harboring platelet-specific PF4-Cre (PF4-Cre+Sdf1flox/flox) and Rosa-CreERT2 (Rosa-Cre+Sdf1flox/flox). Mice with endothelial cell (EC)-specific VE-cadherin-CreERT2 were also bred with mice carrying floxed SDF1 receptors Cxcr4 and Cxcr7 (VE-Cad-Cre+Cxcr4flox/floxCxcr7flox/flox) and floxed Mmp14 mice (VE-Cad-Cre+Mmp14flox/flox). Lung morphology was assessed by hematoxylin and eosin (H&E) staining (c), and cellular proliferation was measured by staining incorporation of injected BrdU in the cryosection (d). Scale bar = 50 μm. (e,f) Pulmonary function assay such as inspiratory volume (e) and compliance (f) was performed to compare the respiratory capacity between wild type and PF4-Cre+Sdf1flox/flox mice after sham operation. n = 4 mice in WT group and = 3 mice for PF4-Cre+Sdf1flox/flox group. P = 0.38 (e) and 0.98 (f) between WT and mutant groups. Error bar denotes standard error of mean (s.e.m.), and line represents mean for panels e,f. All individual groups were compared by unpaired two tail t-test throughout this study.
Supplementary Figure 2 Contribution of platelets in driving lung alveolar regeneration induced by PNX.
(a) Global view of BrdU immunostaining in the residual lung lobe at after PNX. Cellular proliferation was analyzed by staining of BrdU incorporation. Five fields were randomly chosen to quantify incorporation of BrdU as indicated; Scale bar = 50 μm. (b) Depletion of platelets in adult mice by injection of rat anti-mouse CD41 monoclonal antibody (mAb). Platelet counts in mice were determined at indicated time points after injection of CD41 mAb and same amounts of rat IgG control. Injection of CD41 mAb caused statistically lower number of circulating platelet count than IgG treated groups in all tested time points after injection; ∗, P < 0.01, compared with IgG-treated mice; n = 6 mice per group. (c,d) Proliferation of surfactant protein C (SFTPC)+ type 2 alveolar epithelial cells (AEC2s) (c) and regrowth of lung mass (d) in platelet-depleted and control mice after PNX. Representative immunostaining image is shown, and quantification of proliferating AEC2s and VE-cadherin+ PCECs is presented in Fig. 1. n = 5 mice in all groups, and P = 0.000665 between IgG and CD41 mAb (PNX group). Scale bar = 50 μm. (e) PNX caused enrichment of SDF1+CD41+ platelets on VE-cadherin+ pulmonary capillary endothelial cells (PCECs) on day 5. By contrast, sham-operated mice receiving only thoracotomy exhibited lower platelet deposition on PCECs; Scale bar = 50 μm. Error bar depicts s.e.m., and line stands for mean for panels b,d. Statistical difference between individual groups was determined by unpaired two tail t-test throughout this study.
Supplementary Figure 3 Genetic approaches to illustrate cell type-specific contribution of SDF1 in promoting lung regeneration.
(a) Floxed Sdf1 mice were crossed with myeloid cell-specific LysM-Cre, VE-cadherin-CreETR2 mice to generate mice lacking Sdf1 in myeloid and endothelial cells (ECs). Resultant myeloid- and EC-specific Sdf1 knockout mice (LysM-Cre+Sdf1f/f and VE-Cad-CreER+Sdf1f/f) underwent PNX, and lung regenerative responses were tested and compared. (b–j) Regeneration parameters were compared between wild type, myeloid- and EC-specific Sdf1 knockout mice (LysM-Cre+Sdf1f/f and VE-Cad-CreER+Sdf1f/f) after PNX, including increase in right lung weight (b) and volume (c), proliferation/BrdU incorporation of PCECs and AEC2s (d–g), recovery of type 1 alveolar epithelial cell (AEC1) (h), and restoration of pulmonary function (i,j). In (b) and (c), n = 4 mice in WT group and 3 animals for both LysM-Cre+Sdf1f/f and VE-Cad-CreER+Sdf1f/f groups. In (e) and (f), n = 4 animals in all tested genotypes. In (i) and (j), n = 4 mice in WT and 3 mice in LysM-Cre+Sdf1f/f groups. P = 0.48 (i) and 0.86 (j) between these two groups; Scale bar = 50 μm. Error bar defines s.e.m., and line represents mean for panels b,c,e,f,i,j. Statistical difference between individual groups was assessed by unpaired two tail t-test throughout this study.
Supplementary Figure 4 Strategy to compare paracrine factor deployment and coagulation in wild type (Sdf1+/+) and Sdf1-deficient (Sdf1−/−) platelets.
(a) Mouse line with platelet/platelet progenitor-specific platelet factor 4 promoter-driven Cre (PF4-Cre) was crossed with Sdf1loxP/loxP mice to delete Sdf1 in platelets (Sdf1ΔPLT/ΔPLT). Control and Sdf1-deficient (Sdf1−/−) platelets were isolated from wild type and Sdf1ΔPLT/ΔPLT mice and treated with agonists. Subsequent deployment of angiogenic factors, mobilization of P-selectin was determined in isolated platelets, and accumulation of Gr1+CD45+ myeloid cells was measured in control and Sdf1ΔPLT/ΔPLT mice after PNX. (b,c) Protein levels of VEGF164 (a), angiopoietin2 (b), and FGF-2 (c) in supernatant of WT and Sdf1−/− platelets after ADP stimulation. Protein amount was determined by immunoblot (R&D and Abcam). Platelets were stimulated with 10 μmol/L ADP. (b) n = 4 mice in both groups, P = 0.74 between VEGF levels of both groups and = 0.67 between angiopoietin2 levels of two genotypes. (d) Surface mobilization of P-selectin in wild type and Sdf1−/− platelets was determined by flow cytometry after stimulation of ADP and 0.1 U/mL thrombin. (e) Pulmonary accumulation of Gr1+CD45+ myeloid cells in control and Sdf1ΔPLT/ΔPLT mice after PNX was measured by flow cytometry and the percentage of myeloid cells in the lungs was compared. (f,g) Protein level of tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor1 (PAI1) in supernatant of Sdf1+/+ and Sdf1-deficient (Sdf1−/−) platelets. Platelet activation and protein detection was performed as panels c (Abcam and R&D). n = 4 animals in all groups shown in (f and g), P = 0.95 (f) and 0.47 (g) between two genotypes. (h,i) Plasma level of tPA and PAI1 was also measured in control and Sdf1ΔPLT/ΔPLT mice. n = 4 mice in all groups presented, P = 0.34 (h) and 0.83 (i) between two genotypes. (j,k) Formation of fibrin clots in the lungs was determined after both PNX and lung injury caused by ischemia-reperfusion (I/R). Fibrin deposition in individual mice after 150 min I/R procedure was determined by immunoblot analysis of fibrin β-chain extracted from lung lysates (antibody 350 from American Diagnostica). Mouse left lung was subjected to 30 min ischemia by clamping the left lung hilum and then reperfused for 120 min after removing vascular clamp. Protein level of β-actin in the lung lysates after I/R was measured to control for equal amounts of sampling loading. Procedure of mouse lung I/R and extraction of fibrin clot in the lungs was described in ‘Methods’. In the shown immunoblot image, each lane describes individual mouse. Utilized mice were from two cohorts. Error bar indicates s.e.m., and line stands for mean for panels f,g,h,i. Unpaired two tail t-test was used to determine statistical difference between groups.
Supplementary Figure 5 Inflammatory and thrombotic responses in Thpo−/− mice transplanted with wild type and Sdf1-deficient platelets.
(a) Activation of fibroblast cells in Thpo−/− mice after PNX and transplanted with platelets. Protein level of fibroblast cell marker desmin was tested by immunostaining in recipient Thpo−/− mice after PNX and platelet transplantation. Mouse lungs after intratracheal injection of Bleomycin (I.T. Bleo) at day 21 was also employed as positive control. Scale bar = 50 μm. (b) Approaches to compare inflammatory and thrombotic responses in the lungs of pneumonectomized Thpo−/− mouse after intravascular transfusion of Sdf1+/+ and Sdf1−/− platelets. Sdf1+/+ and Sdf1−/− platelets were infused into Thpo−/− mice via jugular vein, respectively. (c) Comparable extent of pulmonary recruitment and distribution of infused Sdf1+/+ and Sdf1−/− platelets. Recruitment of CD41+Sdf1+/+ and Sdf1−/− platelets in the lung capillary was assessed by quantifying association with VE-cadherin+ PCECs; n = 5 mice. (d,e) Pulmonary accumulation of Gr1+CD45+ myeloid cells in Thpo−/− mice transplanted with Sdf1+/+ and Sdf1−/− platelets. Flow cytometry was used to determine the percentage of Gr1+CD45+ myeloid cells in the lungs after PNX. n = 4 mice in both groups and P = 0.83 between two groups. (f) Fibrin deposition in the lungs of Thpo−/− mice transplanted with Sdf1+/+ and Sdf1−/− platelets. Recipient mice underwent PNX or acute lung I/R injury (150 min). Deposition of fibrin clots was assessed by immunoblot of fibrin β–chain. In the presented immunoblot image, each lane indicates sample from individual mouse. Error bar stands denotes s.e.m., and line represents mean for panel e. Statistical difference between groups was assessed by unpaired two tail t-test.
Supplementary Figure 6 Characterization of isolated surface proteins of mouse PCECs and inducible deletion of Mmp14 specifically in ECs of adult mice.
(a) Mouse PCEC membrane proteins were biotinylated by performing pulmonary perfusion with EZ-Link Sulfo-NHS-LC-Biotin (Pierce) that labels protein free amine groups. Biotinylated proteins were isolated by streptavidin conjugated beads. Immunoblot was used to analyze isolated endothelial surface antigens of PCECs, including vascular endothelial cell receptor 2 (VEGFR2), platelet endothelial cell adhesion molecule-1 (PECAM-1), intercellular adhesion molecule-1 (ICAM-1), angiotensin converting enzyme (ACE), integrin αv,α5,β1,β 3, E-cadherin and epithelial cell adhesion molecule (EPCAM). Cytoplasmic proteins such as β-actin and α-tubulin were also tested by immunoblot to exclude the contamination of non-membrane proteins in isolated PCEC membrane fractions. (b–d) Endothelial cell (EC)-specific inducible deletion of alveologenic paracrine factor Mmp14 in adult mice. VE-cadherin-CreETR2/Cdh5-PAC-CreETR2 mice were crossed with mice carrying floxed Mmp14. Deletion efficiency of target genes was measured by immunoblot in isolated PCECs and quantified after normalization to protein level of β-actin. n = 6 mice per group. (e,f) Proliferation of AEC2s and PCECs was tested in Thpo−/− mice after injection of MMP14 downstream effector EGF. n = 4 animals in all tested groups, and P = 0.002 (e) and 0.012 (f). In the presented immunoblot image, each lane indicates individual mouse. All mice were from two different cohorts. Error bar stands for s.e.m., and line represents mean for panels d–f. Statistical difference between groups was determined by unpaired two tail t-test.
(a–d) Proliferation of SFTPC+ AEC2s and VE-cadherin+ PCECs in AMD3100-treated mice after PNX was determined. CXCR4 antagonist AMD3100 was subcutaneously injected into wild type mice at dose of 2 mg/kg every two days, and proliferation was assessed by staining for BrdU incorporation, and proliferation of AEC2s and PCECs was determined by co-staining with SFTPC (a) and VE-cadherin flow cytometry (c). n = 4 animals in all presented groups, and P = 0.0042 (b) and 0.0051 (d). Scale bar = 50 μm. (e) Restoration of gas exchange function in mice after PNX after treatment of AMD3100. n = 4 animals in both groups, P = 0.043. Error bar describes for s.e.m., and line represents mean for panels b–e. Unpaired two tail t-test was used to determine statistical difference between groups.
Supplementary Figure 8 Inducible endothelial cell (EC)-specific deletion of SDF1 receptors Cxcr4 and Cxcr7 in adult mice.
(a,b) VE-cadherin-CreETR2/Cdh5-PAC-CreETR2 mice were crossed with mice carrying floxed Cxcr4 and Cxcr7. Tamoxifen treatment in the resultant mice led to EC-specific deletion of Cxcr4 (a) and Cxcr7 (b) in adult mice. Deletion efficiency of target genes was measured by immunoblot in isolated PCECs after normalization to β-actin. Representative blot image is shown with corresponding protein markers. n = 6 per mouse group, and CXCR4 and CXCR7 protein level was reduced by 92% and 94%, compared to Cxcr4+/+ and Cxcr7+/+ littermate. (c) Activation of Akt in PCECs of indicated mouse groups at day 7 after PNX. n = 4 mice in all groups, and P = 0.00012 (control versus Cxcr4iΔEC/iΔEC), P = 0.00002 (control versus Cxcr4iΔEC/iΔECCxcr7iΔEC/iΔEC). In the presented immunoblot image, each lane indicates individual mouse. Error bar represents s.e.m., and line stands for mean in all panels. Statistical difference between groups was assessed by unpaired two tail t-test.
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Rafii, S., Cao, Z., Lis, R. et al. Platelet-derived SDF-1 primes the pulmonary capillary vascular niche to drive lung alveolar regeneration. Nat Cell Biol 17, 123–136 (2015). https://doi.org/10.1038/ncb3096
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