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Sequential addition of reprogramming factors improves efficiency

Nature Cell Biology volume 15, pages 725–727 (2013)Cite this article

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Addition of a specific set of transcription factors reprograms somatic cell nuclei to a pluripotent state. Sequential addition of these factors, rather than the simultaneous exposure used in standard protocols, improves reprogramming efficiency. This sequential method favours a transition through a state with enhanced mesenchymal characteristics before driving an epithelial transformation on the way to the pluripotent state.

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Figure 1: Heterogeneous fibroblast cultures often serve as the target population for reprogramming to the pluripotent state.

References

  1. Takahashi, K. & Yamanaka, S. Cell 126, 663–676 (2006).

    Article  CAS  Google Scholar 

  2. Liu, X. et al. Nat. Cell Biol. 15, 829–838 (2013).

    Article  CAS  Google Scholar 

  3. Wang, T. et al. Cell Stem Cell 9, 575–587 (2011).

    Article  CAS  Google Scholar 

  4. Buganim, Y. et al. Cell 150, 1209–1222 (2012).

    Article  CAS  Google Scholar 

  5. Golipour, A. et al. Cell Stem Cell 11, 769–782 (2012).

    Article  CAS  Google Scholar 

  6. Stadtfeld, M., Maherali, N., Breault, D. T. & Hochedlinger, K. Cell Stem Cell 2, 230–240 (2008).

    Article  CAS  Google Scholar 

  7. Polo, J. M. et al. Cell 151, 1617–1632 (2012).

    Article  CAS  Google Scholar 

  8. Sridharan, R. & Plath, K. Cell Stem Cell 2, 295–297 (2008).

    Article  CAS  Google Scholar 

  9. Carey, B. W. et al. Proc. Natl Acad. Sci. USA 106, 157–162 (2009).

    Article  CAS  Google Scholar 

  10. Yamanaka, S. Nature 460, 49–52 (2009).

    Article  CAS  Google Scholar 

  11. Samavarchi-Tehrani, P. et al. Cell Stem Cell 7, 64–77 (2010).

    Article  CAS  Google Scholar 

  12. Li, R. et al. Cell Stem Cell 7, 51–63 (2010).

    Article  CAS  Google Scholar 

  13. Miyanari, Y. & Torres-Padilla, M. E. Nature 483, 470–473 (2012).

    Article  CAS  Google Scholar 

  14. Radzisheuskaya, A. et al. Nat. Cell Biol. 15, 579–590 (2013).

    Article  CAS  Google Scholar 

  15. Soufi, A., Donahue, G. & Zaret, K. S. Cell 151, 994–1004 (2012).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Y. Xie and W. E. Lowry are in the Department of Molecular Cell and Developmental Biology, X. Gaeta, UCLA, 621 Charles Young Drive South Los Angeles, California 90095, USA,

    X. Gaeta, Y. Xie & W. E. Lowry

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  1. X. Gaeta
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  2. Y. Xie
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  3. W. E. Lowry
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Correspondence to W. E. Lowry.

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The authors declare no competing financial interests.

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Gaeta, X., Xie, Y. & Lowry, W. Sequential addition of reprogramming factors improves efficiency. Nat Cell Biol 15, 725–727 (2013). https://doi.org/10.1038/ncb2800

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