Supplementary Figure 5 : Cardiomyocyte proliferation and death in G2 hearts.

From: Role of telomere dysfunction in cardiac failure in Duchenne muscular dystrophy

Supplementary Figure 5

(a) Representative image (red Ki67; blue DAPI-stained nuclei, and green immunofluorescence of cardiac Troponin T reveals striated cardiomyocytes) used to assess cardiac proliferation measurements. (b) No major differences on cardiomyocytes proliferation are detected by Ki67 (left graph) and phospho-Histone-3 (right graph) analysis. A total of 8–10 hearts per genotype were analyzed. Two-tailed, unpaired Student’s  t-test, P>0.08 (n.s.). (c) Representative image (green TUNEL; blue DAPI-stained nuclei, and red immunofluorescence of cardiac Troponin T reveals striated cardiomyocytes) used to assess cardiac apoptosis measurements. (d) Increase on cardiomyocytes apoptosis is detected by TUNEL (left graph) and cleaved caspase-3 (right graph) analysis. Data are represented as mean ± s.e.m. (n = 3); Two-tailed, unpaired Student’s  t-test, P values are indicated. Source data are shown in Supplementary Table S1.