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Rab and actomyosin-dependent fission of transport vesicles at the Golgi complex

Nature Cell Biology volume 12, pages 645–654 (2010)Cite this article

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Abstract

Trafficking between membrane compartments is a characteristic of eukaryotic cells and relies on transport carriers that bud and fission from a donor membrane, before being transported and fusing with the correct acceptor compartment. Rab GTPases ensure specificity and directionality of trafficking steps by regulating the movement of transport carriers along cytoskeletal tracks, and the recruitment of tethering factors required for the docking and fusion processes. Here we show that Rab6, a Golgi-associated Rab, forms a complex with myosin II, contributes to its localization at the Golgi complex and, unexpectedly, controls the fission of Rab6 vesicles. Inhibition of either Rab6 or myosin II function impairs both the fission of Rab6 transport carriers from Golgi membranes and the trafficking of anterograde and retrograde cargo from the Golgi. These effects are consistent with myosin II being an effector of Rab6 in these processes. Our results provide evidence that the actomyosin system is required in vesicle biogenesis at the Golgi, and uncover a function for Rab GTPases in vesicle fission.

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Figure 1: Rab6 is involved in fission of the Rab6-positive transport carriers from the Golgi.
Figure 2: Active Rab6 interacts directly with myosin II and contributes to myosin II localization to the Golgi complex.
Figure 3: Inhibition of myosin II function inhibits fission of Rab6 transport carriers from the Golgi.
Figure 4: Transient F-actin accumulation is associated with Rab6 transport carrier fission at the Golgi.
Figure 5: Reactivation of the myosin II activity leads to membrane fission at membrane bulges enriched in Rab6, myosin II and F-actin.
Figure 6: Rab6-positive tubules that appear after myosin II inhibition represent unfissioned Rab6 transport carriers.
Figure 7: Rab6 and myosin II functions are required for efficient transport of both anterograde and retrograde cargoes from the Golgi.

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Acknowledgements

We thank R.S. Adelstein, P. Benaroch, A. Burgess, P. Chavrier, D. Dambournet, V. Doye, M. Heuzé, F. Jollivet, A-M. Lennon, G. Montagnac, F. Perez, J. Saraste, J. Stow and J. Young for providing reagents and plasmids; A. Paoletti and P. Tran for the use of their spinning disk confocal microscope; V. Fraisier, J-B. Sibarita and the staff imagerie for their expertise in microscopy. We thank P. Bassereau, Y. Bellaiche, E. Crowell, A-M. Lennon, K. Schauer, P. Tran and J. Young for critical reading of the manuscript. This work has been supported by the Institut Curie, the CNRS, the GenHomme Network Grant 02490-6088 to Hybrigenics and Institut Curie, the Agence Nationale pour la Recherche (grants ANR BLAN05 0107 and ANR BLAN06-3-139786 to BG and ANR JC07-188506 to AE), the Association pour la Recherche sur le Cancer (ARC grant 1095 to SML).

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  1. A. Echard

    Present address: Current address: Institut Pasteur, CNRS URA2582, Membrane traffic and cell division Lab., 28 rue du Dr. Roux, 75015 Paris Cedex 15, France.,

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  1. Institut Curie, CNRS UMR144, Molecular mechanisms of intracellular transport Lab, 26 rue d'Ulm, 75248, Paris Cedex 05, France

    S. Miserey-Lenkei, G. Chalancon, S. Bardin, B. Goud & A. Echard

  2. Hybrigenics SA, 3-5 impasse Reille, 75014, Paris, France

    E. Formstecher

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Contributions

S.M.L., B.G. and A.E. designed the experiments; S.M.L., G.C., S.B., E.F. and A.E. did the experimental work; S.M.L., G.C., B.G. and A.E. analysed the experiments; S.M.L., B.G. and A.E. wrote the manuscript.

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Miserey-Lenkei, S., Chalancon, G., Bardin, S. et al. Rab and actomyosin-dependent fission of transport vesicles at the Golgi complex. Nat Cell Biol 12, 645–654 (2010). https://doi.org/10.1038/ncb2067

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