Letter | Published:

MesP1 drives vertebrate cardiovascular differentiation through Dkk-1-mediated blockade of Wnt-signalling

Nature Cell Biology volume 10, pages 338345 (2008) | Download Citation

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Abstract

ES-cell-based cardiovascular repair requires an in-depth understanding of the molecular mechanisms underlying the differentiation of cardiovascular ES cells. A candidate cardiovascular-fate inducer is the bHLH transcription factor MesP11,2. As one of the earliest markers, it is expressed specifically in almost all cardiovascular precursors and is required for cardiac morphogenesis2,3. Here we show that MesP1 is a key factor sufficient to induce the formation of ectopic heart tissue in vertebrates and increase cardiovasculogenesis by ES cells. Electrophysiological analysis showed all subtypes of cardiac ES-cell differentiation4. MesP1 overexpression and knockdown experiments revealed a prominent function of MesP1 in a gene regulatory cascade, causing Dkk-1-mediated blockade of canonical Wnt-signalling. Independent evidence from ChIP and in vitro DNA-binding studies, expression analysis in wild-type and MesP knockout mice, and reporter assays confirm that Dkk-1 is a direct target of MesP1. Further analysis of the regulatory networks involving MesP1 will be required to preprogramme ES cells towards a cardiovascular fate for cell therapy and cardiovascular tissue engineering. This may also provide a tool to elicit cardiac transdifferentiation in native human adult stem cells.

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Acknowledgements

We are very grateful for expert technical assistance from Christiane Gross who is funded by the Deutsche Forschungsgemeinschaft (DFG; FR 705/11-3) and the Fritz-Bender-Stiftung. We are also very grateful to Frank Ebel (Max-von-Pettenkofer-Institut, Munich) for helping with the laser scanning microscopy experiments. R.D. is funded exclusively by the DFG (FR 705/11-3). C.B. and F.S. is funded by the FöFoLe program of the LMU Munich. H.L. is supported by an Emmy-Noether Fellowship of the DFG. Additional funding was granted by the Helmut Legalotz-Stiftung for FACS and consumables. We thank Christof Niehrs for the mouse Dkk-1 in situ probe and Yumiko Saga for providing us with the MesP1/2 dK.O. embryos.

Author information

Affiliations

  1. Medizinische Klinik und Poliklinik I, Klinikum Großhadern der LMU, D-81377 München, Germany.

    • R. David
    • , C. Brenner
    • , F. Schwarz
    • , S. Brunner
    •  & W.-M. Franz
  2. Lehrstuhl für Pharmakologie und Toxikologie der Universität Erlangen, D-91054 Erlangen, Germany

    • J. Stieber
  3. GSF - National Research Center for Environment and Health, D-85764 Neuherberg, Germany.

    • M. Vollmer
    •  & H. Lickert
  4. Adolph-Butenandt-Institut der LMU, D-80336 München, Germany.

    • E. Mentele
    •  & R. Rupp
  5. Pathologisches Institut der LMU, D-80337 München, Germany.

    • J. Müller-Höcker
  6. Division of Cellular & Molecular Toxicology, Biological Safety Research Center, National Institute of Health Sciences 1-18-1 Kamiyohga, Setagaya-ku, Tokyo 158-8501, Japan.

    • S. Kitajima

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Contributions

R. D. designed the experiments together with W.-M. F. and performed promoter studies; J. M.-H. perfromed the electron mcroscoy analyses; R. R., R. D. and E. M. performed the Xenopus experiments; R. D. and C. B. performed the molecular cloning and ES cell experiments, and RT-PCR; C. B., F. S. and S. B. performed the FACS and immunostaining; J. S. performed the electrophysiological studies; H. L., M. V. and S. K. performed the wild-type and knockout in situ hybridization studies.

Corresponding authors

Correspondence to R. David or W.-M. Franz.

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DOI

https://doi.org/10.1038/ncb1696

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