In contrast to animal and fungal cells, green plant cells contain one or multiple chloroplasts, the organelle(s) in which photosynthetic reactions take place. Chloroplasts are believed to have originated from an endosymbiotic event and contain DNA that codes for some of their proteins. Most chloroplast proteins are encoded by the nuclear genome and imported with the help of sorting signals that are intrinsic parts of the polypeptides. Here, we show that a chloroplast-located protein in higher plants takes an alternative route through the secretory pathway, and becomes N-glycosylated before entering the chloroplast.
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We thank A. Kraut for technical assistance in the transient expression of GFP fusions in plant cells; L. Faye for the gift of antibodies against xylose and fucose residues; C. Robinson for helpful discussion; and J. Brangeon and R. Boyer for technical help in the immunocytochemistry experiments. The authors are grateful to B. Martin for critical reading of the manuscript. This work was supported by grants from the Swedish National Research Council, FORMAS, Wallenberg and Kempe Foundations, and the Swedish Foundation for Strategic Research.
The authors declare no competing financial interests.
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Villarejo, A., Burén, S., Larsson, S. et al. Evidence for a protein transported through the secretory pathway en route to the higher plant chloroplast. Nat Cell Biol 7, 1224–1231 (2005). https://doi.org/10.1038/ncb1330
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