Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Brief Communication
  • Published:

Directionality of F-actin cables changes during the fission yeast cell cycle

Nature Cell Biology volume 7, pages 916–917 (2005)Cite this article

Abstract

Longitudinal F-actin cables are thought to be important for transporting materials for polarized cell growth in fission yeast. We show that most F-actin in the cables is oriented such that the barbed end faces the nearest cell tip during interphase; however, this directionality is reversed during mitosis. These orientations of F-actin ensure proper transport of materials to growing sites during these cell-cycle stages.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Ultrastructure of F-actin cables and orientation of F-actin in cdc25 cells.
Figure 2: Recovery of cables after Lat-A treatment.

Similar content being viewed by others

References

  1. Marks, J. & Hyams, J. S. Eur. J. Cell Biol. 39, 27–32 (1985).

    Google Scholar 

  2. Arai, R. & Mabuchi, I. J. Cell Sci. 115, 887–898 (2002).

    CAS  PubMed  Google Scholar 

  3. Arai, R., Nakano, K. & Mabuchi, I. Eur. J. Cell Biol. 76, 288–295 (1998).

    Article  CAS  Google Scholar 

  4. Pelham, R. J. Jr & Chang, F. Nature Cell Biol. 3, 235–244 (2001).

    Article  CAS  Google Scholar 

  5. Ishikawa, H., Bischoff, R. & Holtzer, H. J. Cell Biol. 43, 312–328 (1969).

    Article  CAS  Google Scholar 

  6. Lowey, S. et al. J. Mol. Biol. 42, 1–29 (1969).

    Article  CAS  Google Scholar 

  7. Russell, P. & Nurse, P. Cell 45, 145–153 (1986).

    Article  CAS  Google Scholar 

  8. Evangelista, M., Zigmond, S. & Boone, C. J. Cell Sci. 116, 2603–2611 (2003).

    Article  CAS  Google Scholar 

  9. Feierbach, B. & Chang, F. Curr. Biol. 11, 1656–1665 (2001).

    Article  CAS  Google Scholar 

  10. Nakano, K. et al. J. Cell Sci. 115, 4629–4639 (2002).

    Article  CAS  Google Scholar 

  11. Feierbach, B., Verde, F. & Chang, F. J. Cell Biol. 165, 697–707 (2004).

    Article  CAS  Google Scholar 

  12. Reck-Peterson, S. L. et al. Biochim. Biophys. Acta 1496, 36–51 (2000).

    Article  CAS  Google Scholar 

  13. Motegi, F., Arai, R. & Mabuchi, I. Mol. Biol. Cell 12, 1367–1380 (2001).

    Article  CAS  Google Scholar 

  14. Win, T. Z. et al. J. Cell Sci. 114, 69–79 (2001).

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank J. Usukura and M. Sameshima for help in determining the directionality of F-actin. This study was supported by grants from the Ministry of Education, Science and Culture of Japan.

Author information

Author notes

  1. Tomoko Kamasaki

    Present address: Department of Oral Histology, School of Dentistry, Showa University, Tokyo, 142-8555, Japan

  2. Ritsuko Arai

    Present address: Department of Chemical Genetics, Riken, Wako, 351-0198, Japan

Authors and Affiliations

  1. Division of Biology, Graduate School of Arts and Sciences, University of Tokyo, Tokyo, 153-8902, Japan

    Tomoko Kamasaki, Ritsuko Arai & Issei Mabuchi

  2. Division of Material and Biological Function Sciences, Graduate School of Science, Japan Women's University, Tokyo, 112-8681, Japan

    Tomoko Kamasaki & Masako Osumi

  3. Instistute of Medical Mycology, Teikyo University, Tokyo, 192-0395, Japan

    Masako Osumi

Authors
  1. Tomoko Kamasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ritsuko Arai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Masako Osumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Issei Mabuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Issei Mabuchi.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Information

Supplementary figures S1 and S2, supplementary table S1 and supplementary methods (PDF 344 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kamasaki, T., Arai, R., Osumi, M. et al. Directionality of F-actin cables changes during the fission yeast cell cycle. Nat Cell Biol 7, 916–917 (2005). https://doi.org/10.1038/ncb1295

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/ncb1295

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing