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The Diaphanous-related formin dDia2 is required for the formation and maintenance of filopodia

Abstract

Formins have important roles in the nucleation of actin and the formation of linear actin filaments, but their role in filopodium formation has remained elusive. Dictyostelium discoideum Diaphanous-related formin dDia2 is enriched at the tips of filopodia and interacts with profilin II and Rac1. An FH1FH2 fragment of dDia2 nucleated actin polymerization and removed capping protein from capped filament ends. Genetic studies showed that dDia2 is important for cell migration as well as the formation, elongation and maintenance of filopodia. Here we provide evidence that dDia2 specifically controls filopodial dynamics by regulating actin turnover at the barbed ends of actin filaments.

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Figure 1: Domain organization of dDia2, interaction with regulatory proteins and generation of dDia2 mutants.
Figure 2: Interaction of the FH1FH2 construct with actin.
Figure 3: dDia2 localizes to filopodial tips and is required for the formation of filopodia.
Figure 4: Motility and RICM analysis.

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Acknowledgements

We thank J. Segall for discussions and for reading the manuscript, W. Witke for CapG cDNA and D. Rieger and M. Borath for excellent technical assistance. This work was supported by a grant to M.S. from the Deutsche Forschungsgemeinschaft and a grant to J.F. from the Friedrich-Baur-Stiftung.

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Correspondence to Jan Faix.

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Schirenbeck, A., Bretschneider, T., Arasada, R. et al. The Diaphanous-related formin dDia2 is required for the formation and maintenance of filopodia. Nat Cell Biol 7, 619–625 (2005). https://doi.org/10.1038/ncb1266

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