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Functionally distinct kinesin-13 family members cooperate to regulate microtubule dynamics during interphase

Nature Cell Biology volume 7, pages 235–245 (2005)Cite this article

Abstract

Regulation of microtubule polymerization and depolymerization is required for proper cell development. Here, we report that two proteins of the Drosophila melanogaster kinesin-13 family, KLP10A and KLP59C, cooperate to drive microtubule depolymerization in interphase cells. Analyses of microtubule dynamics in S2 cells depleted of these proteins indicate that both proteins stimulate depolymerization, but alter distinct parameters of dynamic instability; KLP10A stimulates catastrophe (a switch from growth to shrinkage) whereas KLP59C suppresses rescue (a switch from shrinkage to growth). Moreover, immunofluorescence and live analyses of cells expressing tagged kinesins reveal that KLP10A and KLP59C target to polymerizing and depolymerizing microtubule plus ends, respectively. Our data also suggest that KLP10A is deposited on microtubules by the plus-end tracking protein, EB1. Our findings support a model in which these two members of the kinesin-13 family divide the labour of microtubule depolymerization.

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Figure 1: Drosophila kinesin-13 proteins affect different parameters of microtubule dynamic instability in interphase S2 cells.
Figure 2: KLP10A and KLP59C localize to microtubule plus ends in interphase S2 cells.
Figure 3: KLP10A moves towards the cell cortex whereas KLP59C moves towards the cell interior.
Figure 4: KLP10A trails the +TIP protein EB1 on most microtubule plus ends.
Figure 5: EB1 is required for proper KLP10A localization at microtubule plus ends.
Figure 6: EB1 binds KLP10A in vitro.
Figure 7: Model for interphase kinesin-13 function.

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Acknowledgements

We thank P. Sampaio and C. Sunkel (Porto, Portugal) for the anti-Mast antibody, E. Ghersi and L. D'adamio (AECOM) for advice on in vitro binding assays, M. Cammer of the AECOM analytical imaging facility for advice on image acquisition and analysis, and H. Sosa (AECOM) for helpful comments on the manuscript. We also thank R. Tsien (UCSD) for providing the mRFP construct, and K. Slep (UCSF) for the APC and some EB1 constructs. This work was supported by grants from the NIH to D.J.S. and R.D.V. V.M. is a Fulbright Fellow and D.J.S. is a Scholar of the Leukemia and Lymphoma Society.

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  1. Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, 10461, New York, USA

    Vito Mennella, Gregory C. Rogers, Daniel W. Buster & David J. Sharp

  2. Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California, San Francisco, 94107, California, USA

    Stephen L. Rogers & Ronald D. Vale

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Correspondence to David J. Sharp.

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Mennella, V., Rogers, G., Rogers, S. et al. Functionally distinct kinesin-13 family members cooperate to regulate microtubule dynamics during interphase. Nat Cell Biol 7, 235–245 (2005). https://doi.org/10.1038/ncb1222

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