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Import of adenovirus DNA involves the nuclear pore complex receptor CAN/Nup214 and histone H1

Nature Cell Biology volume 3, pages 10921100 (2001) | Download Citation

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Abstract

Adenovirus type 2 (Ad2) imports its DNA genome through the nuclear pore complex (NPC) of cells in interphase for viral production. Here we identify the NPC-filament protein CAN/Nup214 as a docking site for incoming Ad2 capsids. Binding to CAN is independent of cytosolic factors. Capsids disassemble at NPCs to free their DNA for import. This process requires binding of nuclear histone H1 to the stably docked capsids and involves H1-import factors, restricting this irreversible process to the proximity of the nucleus. Our results provide a molecular mechanism for disassembly of Ad2 and reveal an unexpected function of histone H1 in virus-mediated DNA import.

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Acknowledgements

We thank P. Groscurth for access to the confocal laser scanning microscope and P. Sonderegger for access to the SMART system, U. Ziegler and P. Cinelli for assistance and U. Aebi, U. Kutay and T. Misteli for comments on the manuscript. Numerous investigators are gratefully acknowledged for their gifts of reagents (and are indicated in the Methods section) and H. van der Velde is acknowledged for preparation of C terminus CAN/Nup214. The work was supported by the Swiss National Science Foundation and the Kanton of Zürich.

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Affiliations

  1. University of Zürich, Institute of Zoology, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland

    • Lloyd C. Trotman
    • , Nicole Mosberger
    • , Robert P. Stidwill
    •  & Urs F. Greber
  2. Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands

    • Maarten Fornerod
  3. ufgreber@zool.unizh.ch

    • Urs F. Greber

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Supplementary information

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    Supplementary Figures

    Figure S1 Microinjected anti-NPC antibodies reduce nuclear import of FITC–BSA–NLS.Figure S2 Histone H1 import factors are required for nuclear import of Ad2–DNA. 

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DOI

https://doi.org/10.1038/ncb1201-1092

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