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Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p

Nature Cell Biology volume 6, pages 405–413 (2004)Cite this article

Abstract

The GTPase Arl3p is required to recruit a second GTPase, Arl1p, to the Golgi in Saccharomyces cerevisiae. Arl1p binds to the GRIP domain, which is present in a number of long coiled-coil proteins or 'golgins'. Here we show that Arl3p is not myristoylated like most members of the Arf family, but is instead amino-terminally acetylated by the NatC complex. Targeting of Arl3p also requires a Golgi membrane protein Sys1p. The human homologues of Arl3p (Arf-related protein 1 (ARFRP1)) and Sys1p (hSys1) can be isolated in a complex after chemical cross-linking. This suggests that the targeting of ARFRP1/Arl3p to the Golgi is mediated by a direct interaction between its acetylated N terminus and Sys1p/hSys1.

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Figure 1: The NatC complex is required for Golgi localization of Imh1p, Arllp and Arl3p.
Figure 2: The N-terminal acetyltransferase activity of NatC is required for Arl3p activity.
Figure 3: The N terminus of Arl3p is acetylated in wild-type, but not in Δmak3, yeast.
Figure 4: Sys1p is required for the Golgi localization of Arl3p.
Figure 5: ARFRP1 and hSys1 are localized to the Golgi.
Figure 6: The N terminus of ARFRP1 is required for recruitment by hSys1.
Figure 7: Coprecipitation of hSys1 with ARFRP1 after chemical cross-linking.

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Acknowledgements

We thank A. Gillingham and D. Görlich for reagents, F. Begum and S. Peak-Chew for mass spectrometry, and M. Freeman and K. Röper for comments on the manuscript.

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  1. Rudy Behnia and Bojana Panic: These authors contributed equally to this work.

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  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    Rudy Behnia, Bojana Panic, James R. C. Whyte & Sean Munro

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Correspondence to Sean Munro.

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Behnia, R., Panic, B., Whyte, J. et al. Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p. Nat Cell Biol 6, 405–413 (2004). https://doi.org/10.1038/ncb1120

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