When two metaphase plates occur in a single cell, they become orientated perpendicularly to each other, seen here in the shape of an 'X'. The image shows a three-dimensional reconstruction of a HeLa cell that was stained for actin (blue), kinetochores (green) and DNA (red). The image was obtained using a laser scanning microscope (Zeiss LSM 510 META), and was rendered using Zeiss LSM Image Browser Software. Scale bar represents 5 μm.