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The APC is dispensable for first meiotic anaphase in Xenopus oocytes

Nature Cell Biology volume 3, pages 83–87 (2001)Cite this article

Abstract

Here we show that segregation of homologous chromosomes and that of sister chromatids are differentially regulated in Xenopus and possibly in other higher eukaryotes. Upon hormonal stimulation, Xenopus oocytes microinjected with antibodies against the anaphase-promoting complex (APC) activator Fizzy or the APC core subunit Cdc27, or with the checkpoint protein Mad2, a destruction-box peptide or methylated ubiquitin, readily progress through the first meiotic cell cycle and arrest at second meiotic metaphase. However, they fail to segregate sister chromatids and remain arrested at second meiotic metaphase when electrically stimulated or when treated with ionophore A34187, two treatments that mimic fertilization and readily induce chromatid segregation in control oocytes. Thus, APC is required for second meiotic anaphase but not for first meiotic anaphase.

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Figure 1: Xenopus oocytes microinjected with anti-FZY antibodies, the recombinant Mad2 protein or a combination of both undergo meiotic maturation and arrest at second meiotic metaphase following progesterone stimulation.
Figure 2: Oocytes microinjected with anti-FZY antibodies, recombinant Mad2, or a combination of both, fail to degrade mitotic cyclins in the first meiotic cell cycle.
Figure 3: Oocytes microinjected with anti-Cdc27 antibodies, destruction-box (D-box) peptide, methylated ubiquitin or xSecurindm mRNA undergo meiotic maturation and arrest at second meiotic metaphase following progesterone stimulation.
Figure 4: Hormone-stimulated oocytes microinjected with anti-FZY antibodies and the Mad2 checkpoint protein fail to degrade mitotic cyclins and to undergo the metaphase II to anaphase II transition upon activation.

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Acknowledgements

We are grateful to H. Zou and M. Kirschner for providing the Securin constructs. We thank Pierre Travo, head of the IFR 24 Integrated Imaging Facility for his constant interest and support. This work was supported by grants from the Association pour la Recherche sur le Cancer and the Ligue Nationale contre le Cancer. We apologise to our collegues whose work has not been cited as a result of referencing constraints.

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  1. CRBM, UPR 1086 CNRS, 1919 route de Mende , Montpellier, cedex 5, 34293, France

    Marion Peter, Anna Castro, Thierry Lorca, Christian Le Peuch, Laura Magnaghi-Jaulin, Marcel Dorée & Jean-Claude Labbé

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  1. Marion Peter
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  3. Thierry Lorca
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Correspondence to Jean-Claude Labbé.

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Peter, M., Castro, A., Lorca, T. et al. The APC is dispensable for first meiotic anaphase in Xenopus oocytes . Nat Cell Biol 3, 83–87 (2001). https://doi.org/10.1038/35050607

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