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Serial bull cloning by somatic cell nuclear transfer

Nature Biotechnology volume 22, pages 693–694 (2004)Cite this article

Abstract

Although the list of species successfully cloned continues to grow, serial cloning has not been reported in species other than the mouse. Here we describe two live births of second-generation clones of a bull. Clones of the first and second generations appear healthy and have normal telomere lengths. Our attempts to produce the third generation of clones were unsuccessful.

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Figure 1: Comparison of telomere lengths in serially cloned bulls.

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Acknowledgements

We would like to thank Hiroshi Yamakuchi for genotyping of the clones, Masumi Sato for pathology, Satoshi Nozaki and Youichi Tsuruta for help with the semen analysis and Marina Julian and Robert Foote for critical reading of the manuscript.

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Authors and Affiliations

  1. Laboratory of Cell Genetics and Embryo Transfer, Kagoshima Prefectural Cattle Breeding Development Institute, Kagoshima, Japan

    Chikara Kubota

  2. Center for Regenerative Biology and Department of Animal Science, University of Connecticut, Storrs, 06269-4243, Connecticut, USA

    Chikara Kubota, X Cindy Tian & Xiangzhong Yang

Authors
  1. Chikara Kubota
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  2. X Cindy Tian
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Correspondence to Xiangzhong Yang.

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The authors declare no competing financial interests.

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Kubota, C., Tian, X. & Yang, X. Serial bull cloning by somatic cell nuclear transfer. Nat Biotechnol 22, 693–694 (2004). https://doi.org/10.1038/nbt975

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