Although the list of species successfully cloned continues to grow, serial cloning has not been reported in species other than the mouse. Here we describe two live births of second-generation clones of a bull. Clones of the first and second generations appear healthy and have normal telomere lengths. Our attempts to produce the third generation of clones were unsuccessful.
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We would like to thank Hiroshi Yamakuchi for genotyping of the clones, Masumi Sato for pathology, Satoshi Nozaki and Youichi Tsuruta for help with the semen analysis and Marina Julian and Robert Foote for critical reading of the manuscript.
The authors declare no competing financial interests.
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Kubota, C., Tian, X. & Yang, X. Serial bull cloning by somatic cell nuclear transfer. Nat Biotechnol 22, 693–694 (2004). https://doi.org/10.1038/nbt975
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