Abstract
In planta expression of recombinant antibodies recognizing pathogen-specific antigens has been proposed as a strategy for crop protection. We report the expression of fusion proteins comprising a Fusarium-specific recombinant antibody linked to one of three antifungal peptides (AFPs) as a method for protecting plants against fungal diseases. A chicken-derived single-chain antibody specific to antigens displayed on the Fusarium cell surface was isolated from a pooled immunocompetent phage display library. This recombinant antibody inhibited fungal growth in vitro when fused to any of the three AFPs. Expression of the fusion proteins in transgenic Arabidopsis thaliana plants conferred high levels of protection against Fusarium oxysporum f.sp. matthiolae, whereas plants expressing either the fungus-specific antibody or AFPs alone exhibited only moderate resistance. Our results demonstrate that antibody fusion proteins may be used as effective and versatile tools for the protection of crop plants against fungal infection.
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Acknowledgements
We thank N. Emans for fluorescence microscopy, S. Hellwig for fermentation of F. graminearum and S. Dorfmueller for valuable discussions. We also thank R. Twyman, P. Christou and N. Emans for critical reading of the manuscript. This project was supported by a state grant of Nordrhein-Westfalen to R.F.
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Peschen, D., Li, HP., Fischer, R. et al. Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen. Nat Biotechnol 22, 732–738 (2004). https://doi.org/10.1038/nbt970
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DOI: https://doi.org/10.1038/nbt970
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