Chloroplastic photorespiratory bypass increases photosynthesis and biomass production in Arabidopsis thaliana

Abstract

We introduced the Escherichia coli glycolate catabolic pathway into Arabidopsis thaliana chloroplasts to reduce the loss of fixed carbon and nitrogen that occurs in C3 plants when phosphoglycolate, an inevitable by-product of photosynthesis, is recycled by photorespiration. Using step-wise nuclear transformation with five chloroplast-targeted bacterial genes encoding glycolate dehydrogenase, glyoxylate carboligase and tartronic semialdehyde reductase, we generated plants in which chloroplastic glycolate is converted directly to glycerate. This reduces, but does not eliminate, flux of photorespiratory metabolites through peroxisomes and mitochondria. Transgenic plants grew faster, produced more shoot and root biomass, and contained more soluble sugars, reflecting reduced photorespiration and enhanced photosynthesis that correlated with an increased chloroplastic CO2 concentration in the vicinity of ribulose-1,5-bisphosphate carboxylase/oxygenase. These effects are evident after overexpression of the three subunits of glycolate dehydrogenase, but enhanced by introducing the complete bacterial glycolate catabolic pathway. Diverting chloroplastic glycolate from photorespiration may improve the productivity of crops with C3 photosynthesis.

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Figure 1: The E.coli glycolate catabolic pathway (red) superimposed on plant photorespiration (black).
Figure 2: Activities of the bacterial enzymes in chloroplast extracts.
Figure 3: Growth parameters of transgenic (DEF, GT-DEF) and wild-type (WT) lines.
Figure 4: Reduction of the photorespiratory flow in transgenic plants (DEF, GT-DEF) compared to the wild type (WT).

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Acknowledgements

This work was supported by a PhD scholarship from the Egyptian government to R.K. and grants from the Deutsche Forschungsgemeinschaft and Bayer CropScience to C.P. Thanks to Martin Parry, Alf Keys, Rainer Häusler, Veronica Maurino, Susanne von Caemmerer, John Evans, Margrit Frentzen, Dagmar Weier, Thomas Rademacher, Burkhard Schmidt and Nikolaus Schlaich for helpful discussion of this work and technical support.

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Authors

Contributions

R.K. established the transgenic lines. R.K. and M.N. conducted most of the physiological experiments. K.T. contributed to physiological and growth measurements. R.B. cloned the genes and established initial transgenic lines. H.-J.H. helped to establish enzymatic assays and metabolite measurements. R.R. established techniques for the estimation of photorespiration. N.S. and B.S. determined the chromosomal T-DNA integration sites. C.P. and F.K. designed the approach. C.P. supervised the work and wrote the manuscript.

Corresponding author

Correspondence to Christoph Peterhänsel.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Fig. 1

Multiplex PCR for the detection of plants containing all five open reading frames for the installation of the E. coli glycolate pathway in Arabidopsis chloroplasts. (PDF 41 kb)

Supplementary Fig. 2

Additional growth parameters of wild-type and transgenic lines. (PDF 16 kb)

Supplementary Fig. 3

Glycine and serine concentrations in leaves of wild-type and transgenic lines. (PDF 10 kb)

Supplementary Fig. 4

Typical measuring profile for the determination of the postillumination CO2 burst (PIB). (PDF 24 kb)

Supplementary Fig. 5

Typical measuring profile for the determination of the CO2 compensation point Γ* and the dark respiration rate in the light (Rd). (PDF 77 kb)

Supplementary Table 1

Genomic integration sites of the T-DNAs containing the listed constructs. (PDF 9 kb)

Supplementary Note

Generation and selection of transgenic lines used in this study. (PDF 15 kb)

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Kebeish, R., Niessen, M., Thiruveedhi, K. et al. Chloroplastic photorespiratory bypass increases photosynthesis and biomass production in Arabidopsis thaliana. Nat Biotechnol 25, 593–599 (2007). https://doi.org/10.1038/nbt1299

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