A fiber-optic biosensor array is described for the simultaneous analysis of multiple DNA sequences. A bundle of optical fibers was assembled with each fiber carrying a different oligonucleotide probe immobilized on its distal end. Hybridization of fluorescently labeled complementary oligonucleotides to the array was monitored by observing the increase in fluorescence that accompanied binding. The approach enables fast (<10 min) and sensitive (10 nM) detection of multiple DNA sequences simultaneously, with the potential for quantitative hybridization analysis.
Subscribe to Journal
Get full journal access for 1 year
only $21.58 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Southern, E.M. 1996. DNA chips: analysing sequence by hybridization to oligonucleotides on a large scale. Trends in Genetics 12: 110–115.
Beattie, K.L., Beattie, W.G., Meng, L., Turner, S.L., Coral-Vazquez, R., et al. 1995. Advances in genosensor research. Molecular Pathology 41: 700–706.
Schena, M., Shalon, D., Davis, R.W. and Brown, P.O. 1995. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270: 467–468.
Stimpson, D.I., Hoijer, J.V., Hsieh, W., Jou, C., Gordon, J., Theriault, T. et al. 1995. Real-time detection of DNA hybridization and melting on oligonucleotide arrays by using optical wave guides. Proc. Natl. Acad. Sci. USA 92: 6379–6383.
Pease, A.C., Solas, D., Sullivan, E.J., Cronin, M.T., Holmes, C.P. and Fodor, S.P.A. 1994. Light-generated oligonucleotide arrays for rapid DNA sequence analysis. Proc. Natl. Acad. Sci. USA 91: 5022–5026.
Maskos, U. and Southern, E.M. 1992. Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ. Nuc. Acids. Res. 20: 1679–1684.
Guo, Z., Guifoyle, R.A., Thiel, A.J., Wang, R. and Smith, L.M. 1994. Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports. Nuc. Acids. Res. 22: 5456–5465.
Kolakowski, B., Battaglini, R., Lee, Y.S., Klironomos, G. and Mikkelsen, S.R. 1996. Comparison of an intercalating dye and an intercalant-enzyme conjugate for DNA detection in a microtiter-based assay. Anal. Chem. 68: 1197–1200.
Nikiforov, T.T., Rendle, R.B., Goelet, P., Rogers, Y., Kotewicz, M.L., Anderson, S. et al. 1994. Genetic bit analysis: a solid phase method for typing single nucleotide polymorphisms. Nucleic Acids Res. 22: 4167–4175.
Zammatteo, N., Girardeaux, C., Delforge, D., Pireaux, J., Remade, J. 1996. Amination of polystyrene microwells: application to the covalent grafting of DNA probes for hybridization assays. Anal. Biochem. 236: 85–94.
Rasmussen, S.R., Larsen, M.R. and Rasmussen, S.E. 1991. Covalent immobilization of DNA onto polystyrene microwells: the molecules are only bound at the 5′ end. Anal. Biochem. 198: 138–142.
Matson, R.S., Rampal, J., Pentoney, S.L., Anderson, P.O. and Coassin, P. 1995. Biopolymer synthesis on polypropylene supports: oligonucleotide arrays. Anal. Biochem. 224: 110–116.
Khrapko, K.R., Lysov, Y.R., Ivanov, I.B., Yershov, G.M., Vasilenko, S.K., Florentiev, V.L. et al. 1991. A method for DNA sequencing by hybridization with oligonucleotide matrix. J. DNA Seq. Map. 1: 375–388.
Eggers, M., Hogan, M., Reich, R.K., Lamture, J., Ehrlich, D., Hollis, M. et al. 1994. A microchip for quantitative detection of molecules utilizing luminescent and radioisotope reporter groups. BioTechniques 17: 516–524.
Kreiner, T. 1996. Rapid genetic sequence analysis using a DNA probe array system. Am. Lab. vol: 39–43.
Kumke, M.U., Li, G., McGowan, L.B., Walker, G.T. and Linn, C.P. 1995. Hybridization of fluorescein-labeled DNA oligomers detected by fluorescence anisotropy with protein binding enhancement. Anal. Chem. 67: 3945–3951.
Piunno, P.A.E., Krull, U.J., Hudson, R.H.E., Damha, M.J. and Cohen, H. 1994. Fiber optic biosensor for fluorimetric detection of DNA hybridization. Anal. Chim. Acta 288: 205–214.
Ito, T., Suzuki, T., Lim, D.K., Wellman, S.E. and Ho, I.K. 1995. A novel quantitative receptor autoradiography and in situ hybridization histochemistry technique using storage phosphor screen imaging. J. Neurosci. Methods 59: 265–271.
Nguyen, Q. and Heffelfinger, D.M. 1995. Imaging and quantitation of chemiluminescence using photoexcitable storage phosphor screen. Anal. Biochem. 226: 59–67.
Graham, C.R., Leslie, D. and Squirrell, D.J. 1992. Gene probe assays on a fibre-optic evanescent wave biosensor. Biosen. Bioelectron. 7: 487–493.
Strachan, N.J.C. and Gray, D.I. 1995. A rapid general method for the identification of PCR products using a fibre-optic biosensor and its application to the detection of Listeria. Lett. App. Microbiol. 21: 5–9.
Warts, H.J., Yeung, D. and Parkes, H. 1995. Real-time detection and quantification of DNA hybridization by an optical biosensor. Anal. Chem. 67: 4283–4289.
Fodor, S.P.A., Rava, R.R., Huang, X.C., Pease, A.C., Holmes, C.P. and Adams, C.L. 1993. Multiplexed biochemical assays with biological chips. Nature 364: 555–556.
Millan, K.M., Saraullo, A. and Mikkelsen, S.R. 1994. Voltammetric DNA biosensor for cystic fibrosis based on a modified carbon paste electrode. Anal. Chem. 66: 2943–2948.
Pandey, P.C. and Weetall, H.H. 1994. Application of photochemical reaction in electrochemical detection of DNA intercalation. Anal. Chem. 66: 1236–1241.
Hashimoto, K., Ito, K. and Ishimori, Y. 1994. Novel DNA sensor for electrochemical gene detection. Anal. Chim. Acta 286: 219–224.
Yamaguchi, S. and Shimomura, T. 1993. Adsorption, immobilization, and hybridization of DNA studied by the use of quartz crystal oscillators. Anal. Chem. 65: 1925–1927.
Tombline, G., Bellizzi, D. and Sgaramella, V. 1996. Heterogeneity of primer extension products in asymmetric PCR is due both to cleavage by a structure-specific exo/endonuclease activity of DNA polymerases and to premature stops. Biochem. 93: 2724–2728.
Saiki, R.K., Walsh, P.S., Levensen, C.H. and Erlich, H.A. 1989. Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes. Prac. Natl. Acad. Sci. USA 86: 6230–6234.
Bronk, K.S., Michael, K.L., Pantano, P. and Walt, D.R. 1995. Combined imaging and chemical sensing using a single optical imaging fiber. Anal. Chem. 67: 2750–2757.
Van Ness, J., Kalbfleisch, S., Petrie, C.R., Reed, M.W., Tabone, J.C. and Vermeulen, N.M.J. 1991. A versatile solid support system for oligodeoxy-nucleotide probe-based hybridization assays. Nucleic Acids Res. 19: 3345–3350.
Munkholm, C., Walt, D.R., Milanovich, F.P. and Klainer, S.M. 1986. Polymer modification of fiber optic chemical sensors as a method of enhancing fluorescence signal for pH measurement. Anal. Chem. 58: 1427–1430.
Walt, D.R. and Agayn, V.I. 1994. The chemistry of enzyme and protein immobilization with gluteraldehyde. Trends Anal. Chem. 13: 425–430.
Kulp, T.J., Gamins, I., Angel, S.M., Munkholm, C. and Walt, D.R. 1987. Polymer immobilized enzyme optrodes for the detection of penicillin. Anal. Chem. 59: 2849–2853.
Menger, F.M., Eliseev, A.V. and Migulin, V.A. 1995. Phosphatase catalysis developed via combinatorial organic chemistry. J. Org. Chem. 60: 6666–6667.
Suh, J., Cho, Y. and Lee, K.J. 1991. Macrocyclic metal complexes built on polyethylenimine. J. Am. Chem. Soc. 113: 4198–4202.
Egger, D., Pasamontes, L., Ostermayer, M. and Bienz, K. 1995. Reverse transcription multiplex PCR for differentiation between polio and enteroviruses from clinical and environmental samples. J. Clin. Microbiol. 33: 1442.
Cronin, M.T., Fucini, R.V., Kirn, S.M., Masino, R.S., Wespi, R.M. and Miyada, C.G. 1996. Cystic fibrosis mutation detection by hybridization to light-generated DNA probe arrays. Human Mutation 7: 244–255.
Tyagi, S. and Kramer, F.R. 1996. Molecular beacons: probes that fluoresce upon hybridization. Nature Biotechnology 14: 303–308.
Yokota, T., Otsuka, T., Mosmann, T., Banchereau, J., DeFrance, T., Blanchard, D. et al. 1986. Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 5894–5898.
About this article
Cite this article
Ferguson, J., Boles, T., Adams, C. et al. A fiber-optic DNA biosensor microarray for the analysis of gene expression. Nat Biotechnol 14, 1681–1684 (1996). https://doi.org/10.1038/nbt1296-1681
The Analyst (2020)
Nucleic acid functionalized fiber optic probes for sensing in evanescent wave: optimization and application
RSC Advances (2019)
Microfluidic chip coupled with optical biosensors for simultaneous detection of multiple analytes: A review
Biosensors and Bioelectronics (2019)
PLOS ONE (2018)