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Establishment of Genetic Linkage by Allele–specific Polymerase Chain Reaction: Application to the Lignin Peroxidase Gene Family of Phanerochaete chrysosporium

Bio/Technology volume 12, pages 1372–1375 (1994)Cite this article

Abstract

Determining linkage is problematic for genes lacking easily identifiable phenotypes and for organisms without well–defined genetic recombination systems. Phanerochaete chrysosporium with its lignin peroxidase (LiP) gene family typifies these difficulties. We describe an experimental approach whereby the segregation of specific alleles is directly monitored during sexual fruiting. The method establishes linkage relationships among genes for which there are no mutations, and it is applicable to a wide range of genes, gene families and organisms. Using this approach, five P. chrysosporium linkage groups were identified. Ten LiP genes were distributed among three of these groups. One co–segregating group contained eight closely linked LiP genes. Another LiP gene was linked to a cellobiohydrolase gene cluster. These genetic linkages were consistent with physical mapping by pulsed field gel electrophoresis. Based on the identification of allelic relationships, a uniform nomenclature for LiP genes is also described.

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Author information

Authors and Affiliations

  1. USDA Forest Service, Forest Products Laboratory, One Gifford Pinchot Dr., Madison, WI, 53705

    Jill Gaskell, Philip J. Kersten & Daniel Cullen

  2. Department of Bacteriology, University of Wisconsin, Madison, WI, 53706

    Philip Stewart & Daniel Cullen

  3. Warnell School of Forest Resources, University of Georgia, Athens, GA, 30602

    Sarah F. Covert

  4. National Institutes of Health, Bethesda, MD, 20892

    Jakob Reiser

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  1. Jill Gaskell
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Correspondence to Daniel Cullen.

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Gaskell, J., Stewart, P., Kersten, P. et al. Establishment of Genetic Linkage by Allele–specific Polymerase Chain Reaction: Application to the Lignin Peroxidase Gene Family of Phanerochaete chrysosporium. Nat Biotechnol 12, 1372–1375 (1994). https://doi.org/10.1038/nbt1294-1372

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