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Rapid Production of Fertile Transgenic Plants of Rye (Secale cereale L.)

Bio/Technology volume 12, pages 1366–1371 (1994)Cite this article

Abstract

We have produced transgenic rye plants by the direct delivery of plasmid DNA, containing the uidA and bar genes, into young embryogenic calli using high velocity microprojectiles. Of the fourteen independent callus lines selected on varying concentrations of phosphinothricin (PPT), the active ingredient of the herbicide Basta, six showed phosphinothricin acetyltransferase (PAT) activity. Plants (R0) were regenerated from two putative transformed lines on medium containing Basta. All of the R0 plants recovered from the two lines showed GUS expression (in leaves, roots, pistils and pollen), PAT activity and resistance to topical application of Basta. The presence of the uidA and bar genes and their integration into nuclear DNA in transformed R0 plants and their R1 progeny was confirmed by Southern analysis. Flowering fertile R0 plants were obtained 8–9 months after the initial culture and bombardment. Male and female transmission of the two genes, which segregated as dominant Mendelian traits in R1 plants, was demonstrated.

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Author notes

  1. Ana M. Castillo

    Present address: Dpto. Genetica y Producción Vegetal, Estación Experimental de Aula Dei, Apdo. 202, Zaragoza, Spain

  2. Indra K. Vasil: Corresponding author.

Authors and Affiliations

  1. Laboratory of Plant Cell and Molecular Biology, University of Florida, 1143 Fifield Hall, Gainesville, FL, 32611-0690

    Vimla Vasil & Indra K. Vasil

Authors
  1. Ana M. Castillo
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  2. Vimla Vasil
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  3. Indra K. Vasil
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Castillo, A., Vasil, V. & Vasil, I. Rapid Production of Fertile Transgenic Plants of Rye (Secale cereale L.). Nat Biotechnol 12, 1366–1371 (1994). https://doi.org/10.1038/nbt1294-1366

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