Abstract
By systematically manipulating promoter and ribosome binding structures, plasmid copy number and the structure of the cholera toxin B (CTB) subunit gene, we were able to develop a plasmid expression system that, when used in conjunction with an optimized growth medium, provided yields of CTB approaching one gram per liter. The CTB protein which was secreted to >95%, could readily be purified from the growth medium of a V. cholerae production strain and was shown to be immunologically indistinguishable from previously used vaccine preparations of native or recombinant CTB.
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Lebens, M., Johansson, S., Osek, J. et al. Large-Scale Production of Vibrio cholerae Toxin B Subunit for Use in Oral Vaccines. Nat Biotechnol 11, 1574–1578 (1993). https://doi.org/10.1038/nbt1293-1574
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DOI: https://doi.org/10.1038/nbt1293-1574