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Large-Scale Production of Vibrio cholerae Toxin B Subunit for Use in Oral Vaccines

Bio/Technology volume 11, pages 15741578 (1993) | Download Citation

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Abstract

By systematically manipulating promoter and ribosome binding structures, plasmid copy number and the structure of the cholera toxin B (CTB) subunit gene, we were able to develop a plasmid expression system that, when used in conjunction with an optimized growth medium, provided yields of CTB approaching one gram per liter. The CTB protein which was secreted to >95%, could readily be purified from the growth medium of a V. cholerae production strain and was shown to be immunologically indistinguishable from previously used vaccine preparations of native or recombinant CTB.

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Author notes

    • M. Lebens

    Corresponding author.

Affiliations

  1. University of Göteborg, Dept. of Medical Microbiology and Immunology, Guldhedsgatan 10, 413 46 Göteborg, Sweden.

    • M. Lebens
    • , S. Johansson
    • , J. Osek
    • , M. Lindblad
    •  & J. Holmgren

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DOI

https://doi.org/10.1038/nbt1293-1574

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