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Use of Living Columns to Select Specific Phage Antibodies

Bio/Technology volume 11, pages 15651569 (1993) | Download Citation

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Abstract

Here we demonstrate that it is possible to confront two recombinant microorganisms in order to select one using the other. We have shown that an epitope derived from p21ras expressed within the outer membrane protein, LamB, can be recognized both by the monoclonal antibody Y13-259, as well as the single chain Fv fragment derived from it. This specificity, which is maintained when the Y13-259 single chain Fv is expressed as a fusion protein with the phage fd gene 3 protein, has allowed us to use the living column of LamB-ras to purify Y13-259 phage from a background of non-binding phage, even at dilutions as high as 10 phage in 1010 irrelevant phage.

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Author information

Author notes

    • Andrew Bradbury
    •  & Antonino Cattaneo

    Corresponding authors.

Affiliations

  1. SISSA, Biophysics Sector, via Beirut 2-4, 34013 Trieste, Italy.

    • Andrew Bradbury
    •  & Antonino Cattaneo
  2. CNR Istituto di Neurobiologia, via Karl Marx 15, 00156 Roma, Italy.

    • Thomas Werge
    •  & Antonino Cattaneo
  3. Società Italiana per la Ricerca Scientifica, Area di Ricerca, Padriciano 99, 34012 Trieste, Italy.

    • Andrew Bradbury
    •  & Lidija Persic

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DOI

https://doi.org/10.1038/nbt1293-1565

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