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Transgenic Corn Plants Expressing MDMV Strain B Coat Protein are Resistant to Mixed Infections of Maize Dwarf Mosaic Virus and Maize Chlorotic Mottle Virus

Bio/Technology volume 11, pages 15591564 (1993) | Download Citation

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Abstract

The maize dwarf mosaic virus strain B (MDMV-B) coat protein (cp) gene was cloned into a monocot expression cassette and introduced into sweet corn cell suspension cultures via particle bombardment or electroporation. Transformed cells were selected on culture media containing 300 mg/l kanamycin, and plants were regenerated. Cells from all transformed lines expressed the cp gene; and one transgenic line synthesized approximately 100–200 μg MDMV-cp per gram fresh weight. Plants regenerated from this line were challenged with a virus inoculum concentration adjusted to produce symptoms in nontransgenic controls at six days post inoculation. In growth chamber studies, the presence of the MDMV-cp provided resistance to inoculations with MDMV-A or MDMV-B and to mixed inoculations of MDMV and maize chlorotic mottle virus.

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Author information

Author notes

    • Lynn E. Murry

    Corresponding author.

Affiliations

  1. Plant Biotechnology, Sandoz Agro, Inc., 975 California Avenue, Palo Alto, CA 94304.

    • Lynn E. Murry
    • , Laurie G. Elliott
    • , Sherry A. Capitant
    • , Joanne A. West
    • , Kimberly K. Hanson
    • , Liliana Scarafia
    • , Scott Johnston
    • , Camille DeLuca-Flaherty
    • , Scott Nichols
    • , Dolores Cunanan
    • , Paul S. Dietrich
    • , Irvin J. Mettler
    • , Steve Dewald
    • , Debra A. Warnick
    • , Carol Rhodes
    • , Ralph M. Sinibaldi
    •  & Karen J. Brunke

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DOI

https://doi.org/10.1038/nbt1293-1559

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