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Transgenic Corn Plants Expressing MDMV Strain B Coat Protein are Resistant to Mixed Infections of Maize Dwarf Mosaic Virus and Maize Chlorotic Mottle Virus

Bio/Technology volume 11, pages 1559–1564 (1993)Cite this article

Abstract

The maize dwarf mosaic virus strain B (MDMV-B) coat protein (cp) gene was cloned into a monocot expression cassette and introduced into sweet corn cell suspension cultures via particle bombardment or electroporation. Transformed cells were selected on culture media containing 300 mg/l kanamycin, and plants were regenerated. Cells from all transformed lines expressed the cp gene; and one transgenic line synthesized approximately 100–200 μg MDMV-cp per gram fresh weight. Plants regenerated from this line were challenged with a virus inoculum concentration adjusted to produce symptoms in nontransgenic controls at six days post inoculation. In growth chamber studies, the presence of the MDMV-cp provided resistance to inoculations with MDMV-A or MDMV-B and to mixed inoculations of MDMV and maize chlorotic mottle virus.

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  1. Lynn E. Murry: Corresponding author.

Authors and Affiliations

  1. Plant Biotechnology, Sandoz Agro, Inc., 975 California Avenue, Palo Alto, CA, 94304

    Lynn E. Murry, Laurie G. Elliott, Sherry A. Capitant, Joanne A. West, Kimberly K. Hanson, Liliana Scarafia, Scott Johnston, Camille DeLuca-Flaherty, Scott Nichols, Dolores Cunanan, Paul S. Dietrich, Irvin J. Mettler, Steve Dewald, Debra A. Warnick, Carol Rhodes, Ralph M. Sinibaldi & Karen J. Brunke

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Murry, L., Elliott, L., Capitant, S. et al. Transgenic Corn Plants Expressing MDMV Strain B Coat Protein are Resistant to Mixed Infections of Maize Dwarf Mosaic Virus and Maize Chlorotic Mottle Virus. Nat Biotechnol 11, 1559–1564 (1993). https://doi.org/10.1038/nbt1293-1559

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