During three consecutive growing seasons, transgenic cucumbers of the cultivar Poinsett 76 possessing the coat protein gene of a non-aphid transmissible strain of cucumber mosaic virus (CMV) were compared with the non-transformed plants of the same cultivar and CMV-resistant Marketmore 76. Unique to this study, a low percentage of CMV-infected cucumbers provided the major source of virus, which was naturally spread by the indigenous aphid populations. Monitoring of test plants by visual inspection and ELISA provided a dynamic evaluation on the resistance of transgenic plants throughout the plant growth cycle. Eight weeks after transplanting, the critical period when plants were bearing fruit, the percentage of infection in transgenic Poinsett 76 and Marketmore 76 plants averaged less than 5%, in contrast to about 72% in non-transformed plants. Trials were terminated 13 weeks after field transplanting, when plants had entered the senescence stage. At this time, ELISA demonstrated an appreciable level of CMV infection in transgenic lines (35%) and a much higher amount in Marketmore 76 (62%), while an average of 85% of the non-transformed Poinsett 76 tested positive. Fruit yields and vegetative growth of transgenic lines averaged better than those of non-transformed plants of the same cultivar.
This is a preview of subscription content, access via your institution
Subscribe to Journal
Get full journal access for 1 year
only $8.25 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Tax calculation will be finalised during checkout.
Get time limited or full article access on ReadCube.
All prices are NET prices.
Powell-Abel, P., Nelson, R.S., De, B., Hoffmann, N., Rogers, S.G., Fraley, R.T. and Beachy, R.N. 1986. Delay of disease development in transgenicplants that express the tobacco mosaic virus coat protein gene. Science 232: 738–743.
Beachy, R.N., Loesch-Fries, S. and Tumer, N.E. 1990. Coat protein-mediated resistance against virus infection. Ann. Rev. Phytopathol. 28: 451–74.
Loesch-Fries, L.S., Merlo, D., Zinnen, X., Burhop, L., Hill, K., Krahn, K., Jarvis, N., Nelson, S. and Halk, E. 1987. Expression of alfalfa mosaic virus RNA 4 in transgenic plants confers virus resistance. EMBO J. 6: 1845–1852.
Nelson, R.S., McCormick, S.M., Delannay, X., Dube, P., Layton, J., Anderson, E.J., Kaniewska, M., Proksch, R.K., Horsch, R.B., Rogers, S.G., Fraley, R.T. and Beachy, R.N. 1988. Virus tolerance, plant growth, and field performance of transgenic tomato plants expressing coat protein from tobacco mosaic virus. Bio/Technology 6: 403–409.
Tumer, N.E., O'Connell, K.M., Nelson, R.S., Sander, P.R., Beachy, R.N., Fraley, R.T. and Shah, D.M. 1987. Expression of alfalfa mosaic virus coat protein gene confers cross-protection in transgenic tobacco and tomato plants. EMBO J. 6: 1181–1188.
Hill, K.K., Jarvis-Egan, N., Halk, E.L., Krahn, K.J., Liao, L.W., Mathewson, R.S., Merlo, D.J., Nelson, S.E., Rashka, K.E. and Loesch-Fries, L.S. 1991. The development of virus-resistant alfalfa, Medicogo sativa L. Bio/Technology 9: 373–377.
Hoekema, A., Huisman, M.J., Molendijk, L., Elzen, P.J.M., Van den and Cornelissen, B.J.C. 1989. Genetic engineering of two commercial potato cultivars for resistance to potato virus X. Bio/Technology 7: 273–278.
Kaniewski, W., Lawson, C., Sammons, B., Haley, L., Hart, J., Delannay, X. and Tumer, N.E. 1990. Field resistance of transgenic russet burbank potato to effects of infection by potato virus X and potato virus Y. Bio/Technology 8: 750–754.
Kawchuk, L.M., Martin, R.R. and McPherson, J. 1990. Resistance in transgenic potato expressing the potato leafroll virus coat protein gene. Mol. Plant Microbe Interact. 3: 301–307.
Lawson, C., Kaniewski, W., Haley, L., Rozman, R., Newell, C., Sanders, P. and Tumer, N.E. 1990. Engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus X and potato virus Y in transgenic russet burbank. Bio/Technology 8: 127–134.
Mackenzie, D.J., Tremaine, J.H. and McPherson, J. 1991. Genetically engineered resistance to potato virus S in potato cultivar russet burbank. Mol. Plant Microbe Interact. 4: 95–102.
Jongedijk, E., De Schutter, A.A.J.M., Stolte, X., Van den Elzen, P.J.M. and Cornelissen, B.J.C. 1992. Increased resistance to potato virus X and preservation of cultivar properties in transgenic potato under field conditions. Bio/Technology 10: 422–429.
Matthews, R.E.F. 1991 Plant Virology. Academic Press, Inc., San Diego,CA.
Kaper, J.M. and Waterworth, H.E. 1981. Cucumoviruses. comparative Diagnosis, Chapter 11, p. 257–332.In: Handbook of Plant Virus Infections. Kurstak, E. (Ed.). Elsevier/North-Holland Biomedical Press, The Netherlands.
Munger, H.M. 1985. Near-isogenic lines of several cucumber varieties. Cucurbit Genetics Cooperative 8: 4–6.
Chee, P.P. and Slightom, J.L. 1991. Transfer and expression of cucumber mosaic virus coat protein gene in the genome of cucumis-sativus. J. Am. Soc. Hortic. Sci. 116: 1098–1102.
Quemada, H., Kearney, C., Gonsalves, D. and Slightom, J.L. 1989. Nucleotide sequences of the coat protein genes and flanking regions of cucumber mosaic virus strains C and WL RNA 3. J. Gen. Virol. 70: 1065–1074.
Quemada, H.D., Gonsalves, D. and Slightom, J.L. 1991. Expression of coat protein gene from cucumber mosaic virus strain C in tobacco: protection against infections by CMV strains transmitted mechanically or by aphids. Phytopathology 81: 794–802.
Namba, S., Ling, K., Gonsalves, C., Gonsalves, D. and Slightom, J.L. 1991. Expression of the gene encoding the coat protein of cucumber mosaic virus (CMV) strain WL appears to provide protection to tobacco plants against infection by several different CMV strains. Gene 107: 181–188.
Nawrocka, B.Z., Eckenrode, C.J., Uyemoto, J.K. and Young, D.H. 1975. Reflective mulches and foliar sprays for suppression of aphid-borne viruses in lettuce. J. Econ. Entomol. 68: 694–698.
Wang, H.L., Gonsalves, D., Provvidenti, R., Zitter, T.A. 1992. Biological and serological properties of four strains of zucchini yellow mosaic virus. Plant Disease 76: 530–535.
Yeh, S.D, Gonsalves, D. and Provvidenti, R. 1984. Comparative studies on host range and serology of papaya ringspot virus and watermelon mosaic virus 1. Phytopathology 74: 1081–1085.
Pierce, L.K. and Wehner, T.C. 1990. Review of genes and linkage groups in cucumber. Hort. Sci. 25: 605–615.
DeZoeten, G.A. 1991. Risk assessment: do we let history repeat itself? Phytopathology 81: 585–586.
Osbourn, J.K., Sakar, S. and Wilson, M.A. 1990. Complementation of coat protein-defective TMV mutants in transgenic tobacco plants expressing TMV coat protein. Virology 179: 921–925.
Edwards, M.C. and Gonsalves, D. 1983. Grouping of seven biologically defined isolates of cucumber mosaic virus by peptide mapping. Phytopathology 73: 1117–1120.
Jefferson, R.A. 1987. Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol. Biol. Reporter 5: 387–405.
Clark, M.F. and Adams, A.N. 1977. Characteristics of the microplate method for enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34: 475–483.
SAS institute 1985. SAS User's Guide: Statistics. Version 5 Edition. SAS Institute, Gary, NC.
About this article
Cite this article
Gonsalves, D., Chee, P., Provvidenti, R. et al. Comparison of Coat Protein-Mediated and Genetically-Derived Resistance in Cucumbers to Infection by Cucumber Mosaic Virus Under Field Conditions with Natural Challenge Inoculations by Vectors. Nat Biotechnol 10, 1562–1570 (1992). https://doi.org/10.1038/nbt1292-1562
This article is cited by