Research Paper | Published:

Enhanced Secretion of Human Nerve Growth Factor from Saccharomyces cerevisiae using an Advanced δ–Integration System

Bio/Technology volume 9, pages 13821385 (1991) | Download Citation

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Abstract

We have designed an advanced δ–integration system (integration of genes into the δ–sequence of yeast retrotransposon Ty) and used it for secretion of human nerve growth factor (hNGF) from Saccharomyces cerevisiae. The expression and secretion of hNGF was directed by the PGK promoter and MFα1 prepro–signal. Using two selectable markers (URA3 and leu2–d), haploid yeast strains were constructed with approximately 20 copies of a δ–integrated hNGF expression cassette on four chromosomes. The strain secreted hNGF at levels 3–4 fold higher than a 2μm–based plasmid. Northern and Western analyses revealed that the oversecretion was caused by an increased amount of mRNA. We also detected an unusual processing of the MFα1 prepro–hNGF fusion protein that required the pep4 mutation. Application of this system for industrial purposes is discussed.

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Author notes

    • Akira Sakai

    Corresponding author.

Affiliations

  1. Laboratory of Molecular Genetics, Mitsubishi Kasei Institute of Life Sciences, 11, Minamiooya, Machida-shi, Tokyo 194, Japan.

    • Akira Sakai
    • , Fumiko Ozawa
    • , Takako Higashizaki
    • , Yuki Shimizu
    •  & Fumio Hishinuma

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DOI

https://doi.org/10.1038/nbt1291-1382

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