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A Streptavidin–Protein a Chimera that allows one–step production of a variety of Specific Antibody Conjugates

Bio/Technology volume 9, pages 1378–1381 (1991)Cite this article

Abstract

We have expressed a gene fusion of streptavidin and the two immunoglobulin G (IgG)–binding domains of protein A in Escherichia coli. The purified chimeric protein had full biotin–binding ability, and bound one IgG molecule per subunit (31.4 kD). The affinity of the chimeric protein both for biotin and IgG can be used to provide antibody molecules with additional recognition capabilities. For example, the chimera will allow any IgG molecule to be used for sensitive biotin–linked detection systems without the need for chemical modification.

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Authors and Affiliations

  1. Department of Molecular and Cell Biology, University of California, and Division of Chemical Biodynamics, Lawrence Berkeley Laboratory, Berkeley, CA, 94720

    Takeshi Sano & Charles R. Cantor

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  1. Takeshi Sano
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  2. Charles R. Cantor
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Sano, T., Cantor, C. A Streptavidin–Protein a Chimera that allows one–step production of a variety of Specific Antibody Conjugates. Nat Biotechnol 9, 1378–1381 (1991). https://doi.org/10.1038/nbt1291-1378

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  • DOI: https://doi.org/10.1038/nbt1291-1378

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