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Secretion of Active Human Tissue Plasminogen Activator from the Filamentous Fungus Aspergillus Nidulans

Bio/Technology volume 5, pages 1301–1304 (1987)Cite this article

Abstract

Strains of the filamentous fungus Aspergillus nidulans transformed with a functional cDNA comprising the entire coding region of human tissue plasminogen activator (t-PA) secrete biologically active protein into the culture medium. Activity is inhibited by treatment with t-PA specific polyclonal antibodies. The protein is correctly processed at the amino terminus and is not excessively glycosylated.

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Author notes

  1. A. Upshall: Corresponding author.

Authors and Affiliations

  1. ZymoGenetics, Inc., 2121 N. 35th Street, Seattle, WA, 98103

    A. Upshall, A. A. Kumar, M. C. Bailey, M. D. Parker, M. A. Favreau, K. P. Lewison, M. L. Joseph, J. M. Maraganore & G. L. McKnight

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  1. A. Upshall
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  2. A. A. Kumar
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  3. M. C. Bailey
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  4. M. D. Parker
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  5. M. A. Favreau
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  6. K. P. Lewison
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  7. M. L. Joseph
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  8. J. M. Maraganore
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  9. G. L. McKnight
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Upshall, A., Kumar, A., Bailey, M. et al. Secretion of Active Human Tissue Plasminogen Activator from the Filamentous Fungus Aspergillus Nidulans. Nat Biotechnol 5, 1301–1304 (1987). https://doi.org/10.1038/nbt1287-1301

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  • DOI: https://doi.org/10.1038/nbt1287-1301

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