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Efficient Plant Regeneration from Rice Protoplasts Through Somatic Embryogenesis

Bio/Technology volume 4, pages 1087–1090 (1986)Cite this article

Abstract

Progress in the genetic manipulation of cereals using protoplast technology has been limited by a lack of reproducible plant regeneration from protoplasts of these important species. Here we report that plant regeneration can be achieved efficiently and reproducibly from protoplasts of rice isolated from cell suspension cultures. Regeneration from 10—20% of protoplast–derived colonies of two varieties was obtained rapidly after direct transfer to a hormone–free medium. Green plants were produced at a high frequency through somatic embryogenesis. The protoplast origin of the regenerated plants is unequivocally demonstrated by the inability of contaminating intact cells to divide.

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Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, Nottingham, U.K.

    Ruslan Abdullah, Edward C. Cocking & John A. Thompson

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  1. Ruslan Abdullah
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  2. Edward C. Cocking
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  3. John A. Thompson
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Abdullah, R., Cocking, E. & Thompson, J. Efficient Plant Regeneration from Rice Protoplasts Through Somatic Embryogenesis. Nat Biotechnol 4, 1087–1090 (1986). https://doi.org/10.1038/nbt1286-1087

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  • DOI: https://doi.org/10.1038/nbt1286-1087

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